Host: Rabbit
Target Protein: SOD2
Immunogen Range: 21-120/222
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 6648
Swiss Prot: P04179
Source: KLH conjugated synthetic peptide derived from human SOD2
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 22
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Horse
Chicken
For research use only. Not intended for diagnostic or therapeutic use.
- Zhang-Jie Yang. et al. SIRT 3 was involved in Lycium barbarum seed oil protection testis from oxidative stress: in?vitro and in?vivo analyses. Pharm Biol. 2021;59(1):1314-1325Read more>>
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Lane 1: Mouse Kindey lysates; Lane 2: Mouse Stomach lysates probed with SIRT4 Polyclonal Antibody, Unconjugated (bs-23402R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SOD2 Polyclonal Antibody, Unconjugated (bs-23402R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SOD2 Polyclonal Antibody, Unconjugated (bs-23402R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SOD2 Polyclonal Antibody, Unconjugated (bs-23402R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SOD2 Polyclonal Antibody, Unconjugated (bs-23402R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SOD2 Polyclonal Antibody, Unconjugated (bs-23402R) at 1:200 overnight at 4°C, DAB staining.
Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Kidney lysates; Lane 3: Mouse Testis lysates; Lane 4: Mouse Liver lysates; Lane 5: Rat Kidney lysates; Lane 6: Rat Testis lysates; Lane 7: Rat Pancreas lysates; Lane 8: Rat Liver lysates probed with SOD2 Polyclonal Antibody, Unconjugated (bs-23402R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.