bs-23407R [Primary Antibody]
NQO1 Polyclonal Antibody
www.biossusa.com
[email protected]
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DATASHEET

Host: Rabbit

Target Protein: NQO1

Immunogen Range: 201-274/274


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 18104

Swiss Prot: Q64669

Source: KLH conjugated synthetic peptide derived from mouse NQO1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq].

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 31


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Mengru Li. et al. The leaves of Scutellaria baicalensis Georgi attenuate brain aging in D-galactose-induced rats via regulating glutamate metabolism and Nrf2 signaling pathway. EXP GERONTOL. 2022 Dec;170:111978Read more>>
  • Wenjie Chen. et al. Transferrin-Targeted Cascade Nanoplatform for Inhibiting Transcription Factor Nuclear Factor Erythroid 2-Related Factor 2 and Enhancing Ferroptosis Anticancer Therapy. ACS APPL MATER INTER. 2023;XXXX(XXX):XXX-XXXRead more>>
VALIDATION IMAGES

Mouse stomach lysates probed with NQO1 Polyclonal Antibody, Unconjugated (bs-23407R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NQO1) Polyclonal Antibody, Unconjugated (bs-23407R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.