bs-2340R [Primary Antibody]
14-3-3 epsilon/YWHAE Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: 14-3-3 epsilon/YWHAE

Immunogen Range: 151-255/255


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7531

Source: KLH conjugated synthetic peptide derived from human 14-3-3E

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 29


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Cow
Sheep
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Human HeLa lysates probed with Rabbit Anti-14-3-3 epsilon Polyclonal Antibody, Unconjugated (bs-2340R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.


Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Placenta tissue lysates; Lane 3: Rat Cerebrum tissue lysates; Lane 4: Rat Testis tissue lysates; Lane 5: Rat Placenta tissue lysates; Lane 6: Human 293T cell lysates; Lane 7: Human A375 cell lysates; Lane 8: Human SW480 cell lysates probed with 14-3-3 epsilon Polyclonal Antibody, Unconjugated (bs-2340R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.