Host: Rabbit
Target Protein: EPO
Immunogen Range: 101-193/193
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 2056
Swiss Prot: P01588
Source: KLH conjugated synthetic peptide derived from human Erythropoietin
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
dot-blot()
Predicted Molecular Weight: 28
Cross Reactive Species:
Human
Rat
Predicted Cross Reactive Species:
Mouse
Dog
Cow
Sheep
Pig
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
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- Li, Xu, et al. "Oxidative stress induces the decline of brain EPO expression in aging rats." Experimental Gerontology (2016).Read more>>
- Desharnais P et al. Detection of Erythropoiesis Stimulating Agents in urine samples using a capillary Western system.(2018) Drug Test AnalRead more>>
- Macias-Velez RJ et al. Intranasal Erythropoietin Protects CA1 Hippocampal Cells, Modulated by Specific Time Pattern Molecular Changes After Ischemic Damage in Rats. J Mol Neurosci. 2019 May 3. Read more>>
- Giraud S et al. The inhibition of eIF5A hypusination by GC7, a preconditioning protocol to prevent brain death\induced renal injuries in a preclinical porcine kidney transplantation model. Am J Transplant. 2020 Dec;20(12):3326-3340.Read more>>
- Pengfei Gao. et al. Pentraxin 3 promotes airway inflammation in experimental asthma. Resp Res. 2020 Dec;21(1):1-1Read more>>
- Yi-Fang Wu. et al. VEGF loaded nanofiber membranes inhibit chronic cerebral hypoperfusion-induced cognitive dysfunction by promoting HIF-1a/VEGF mediated angiogenesis. NANOMED-NANOTECHNOL. 2023 Feb;48:102639Read more>>
- Rafael de Jess Macias-Velez. et al. Intranasal erythropoietin protects granular cells and reduces astrogliosis in the dentate gyrus after ischemic damage, an effect associated with molecular changes in erythropoietin and its receptor. NEUROSCI LETT. 2023 Jun;:137366Read more>>
Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with EPO Polyclonal Antibody, Unconjugated (bs-2343R ) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Formalin-fixed and paraffin embedded rat kidney labeled with Anti-EPO Polyclonal Antibody, Unconjugated (bs-2343R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EPO) Polyclonal Antibody, Unconjugated (bs-2343R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EPO) Polyclonal Antibody, Unconjugated (bs-2343R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Rat Liver tissue lysates; Lane 2: Human HepG2 cell lysates probed with EPO Polyclonal Antibody, Unconjugated (bs-2343R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.