bs-23445R [Primary Antibody]
TGF beta Receptor I Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: TGF beta Receptor I

Immunogen Range: 111-210/503


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7046

Swiss Prot: P36897

Source: KLH conjugated synthetic peptide derived from human TGF beta Receptor I

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for TGF-beta.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF()
ICC(1:100-500)

Predicted Molecular Weight: 55


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat
Dog
Cow
Sheep
Pig
Horse
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Yake Wang. et al. The Upregulation of Leucine-Rich Repeat Containing 1 Expression Activates Hepatic Stellate Cells and Promotes Liver Fibrosis by Stabilizing Phosphorylated Smad2/3. INT J MOL SCI. 2024 Jan;25(5):2735Read more>>
VALIDATION IMAGES

Lane 1: Human K562 cell lysates; Lane 2: Human SH-SY5Y cell lysates probed with TGF beta Receptor I Polyclonal Antibody, Unconjugated (bs-23445R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Human Hela cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human Raji cell lysates; Lane 4: Human U87MG cell lysates ; Lane 5: Human K562 cell lysates probed with TGF beta Receptor I Polyclonal Antibody, Unconjugated (bs-23445R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.