Host: Rabbit
Target Protein: PITX3
Immunogen Range: 231-302/302
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 5309
Source: KLH conjugated synthetic peptide derived from human PITX3
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 32
Cross Reactive Species:
Mouse
Rat
Cow
Predicted Cross Reactive Species:
Human
Dog
Pig
Horse
Chicken
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-PITX3 Polyclonal Antibody (bs-2364R), Unconjugated at 1:200 followed by conjugation to the secondary antibody
Mouse brain lysates 30ug, probed (bs-2364R) at 1:200 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted and observed band size: 33kDa.
Paraformaldehyde-fixed, paraffin embedded Rat eye; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PITX3 Polyclonal Antibody, Unconjugated (bs-2364R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PITX3 Polyclonal Antibody, Unconjugated (bs-2364R) at 1:400 overnight at 4°C, DAB staining.