bs-2382R [Primary Antibody]
ST2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ST2

Immunogen Range: 11-110/556


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 9173

Swiss Prot: Q01638

Source: KLH conjugated synthetic peptide derived from human Interleukin 1 receptor like 1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Receptor for interleukin-33 (IL-33), its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function.

Size: 100ul

Concentration: 1ug/ul

Applications: FCM(1:20-100)

Predicted Molecular Weight: 61


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Yang S-H, Lin J-C, Wu S-Y, Huang K-L, Jung F, et al. (2015) Membrane Translocation of IL-33 Receptor in Ventilator Induced Lung Injury. PLoS ONE 10(3): e0121391.Read more>>
  • Li et al. IL-33 facilitates proliferation of colorectal cancer dependent on COX2/PGE2. (2018) J.Exp.Clin.Cancer.Res. 37:196Read more>>
  • Li et al. IL-33 facilitates proliferation of colorectal cancer dependent on COX2/PGE2. (2018) J.Exp.Clin.Cancer.Res. 37:196Read more>>
VALIDATION IMAGES

Huvec (Positive) and MCF7 (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ST2 Antibody (bs-2382R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).