bs-2385R [Primary Antibody]
KLF5 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: KLF5

Immunogen Range: 61-160/457

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 688

Swiss Prot: Q13887

Source: KLH conjugated synthetic peptide derived from human KLF5

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage: Store at -20°C for 12 months..

Background:

The kinesin motor proteins include at least two forms of conventional kinesin encoded by different genes and designated as ubiquitous kinesin, which is expressed in all cells and tissues, or neuronal kinesin, which is expressed exclusively in neural cells. Kinesin is a microtubule associated protein comprised of three different structural domains. A considerable globular N-terminal domain regulates the hydrolysis of ATP and also microtubule binding while central coiled-coil domains promote heavy chain dimerization. Lastly, small globular C-terminal domains interact with kinesin light chains, membranous organelles and vesicles. Expression of ubiquitous kinesin heavy chain, also designated UKHC, is found subcellularly in areas of heavy vesicular trafficking such as the microtubule pathways of neural cells and also the Golgi of non-neural cell types.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 50

Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Dog
Cow
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhang P, Ha T, Larouche M, Swanson D, Goldowitz D (2015) Kruppel-Like Factor 4 Regulates Granule Cell Pax6 Expression and Cell Proliferation in Early Cerebellar Development. PLoS ONE 10(7): e0134390.Read more>>
VALIDATION IMAGES

L1 rat brain lysates L2 human colon carcinoma lysates probed with Anti KLF5/UKHC Polyclonal Antibody, Unconjugated (bs-2385R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 50kD. Observed band size:50kD.\n


Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti KLF5/UKHC Polyclonal Antibody, Unconjugated (bs-2385R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin-embedded rat brain labeled with Anti-KLF5/UKHC Polyclonal Antibody, Unconjugated(bs-2385R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.