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Host: Rabbit
Target Protein: Aurora B (Thr236)
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 9212
Swiss Prot: Q96GD4
Source: KLH conjugated synthetic peptide derived from human Aurora B around the phosphorylation site of(Thr236)
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100µl
Concentration: 1mg/ml
Applications:
WB(WB=1:500-2000)
ELISA(ELISA=1:5000-10000)
IHC-P(IHC-P=1:100-500)
IHC-F(IHC-F=1:100-500)
IF(IF=1:100-500)
ICC/IF(ICC/IF=1:100-500)
Predicted Molecular Weight: 39
Cross Reactive Species: Human
Predicted Cross Reactive Species:
Mouse
Rat
Cow
For research use only. Not intended for diagnostic or therapeutic use.
25 ug total protein per lane of various lysates (see on figure) probed with phospho-Aurora B (Thr236) polyclonal antibody, unconjugated (bs-24218R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.