bs-2421R [Primary Antibody]
CD158e Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD158e

Immunogen Range: 251-350/432


Clonality: Polyclonal

Isotype: IgG

Swiss Prot: P83555

Source: KLH conjugated synthetic peptide derived from mouse CD158e

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

KIR3DL1 (NKB1, CD158e1) is expressed on a subset of natural killer cells and T cells. NKB1 is a 70 kD member of the immunoglobulin superfamily that is expressed at varying levels among individuals. NKB1 is a type I membrane protein containing two immunoglobulin C2 type domains. The interaction of NKB1 with specific HLA B antigens on a target cell (the HLA Bw4 allele, for example) inhibits cytotoxicity and prevents target cell lysis and death. The interactions between KIR and MHC class I are thought to be important in NK and T cell regulation following antigen stimulation. The absence of ligands for KIRs may lower the threshold for activation through activating receptors and increase inflammation and susceptibility to autoimmune disease.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)

Predicted Molecular Weight: 46


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Raji cell lysates probed with Anti-CD158e Polyclonal Antibody, Unconjugated (bs-2421R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.