bs-2430R [Primary Antibody]
Apelin Receptor Polyclonal Antibody
www.biossusa.com
support@biossusa.com
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Apelin Receptor

Immunogen Range: 52-66/380


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 187

Swiss Prot: P35414

Source: KLH conjugated synthetic peptide derived from human Apelin receptor (cytoplasmic domain)

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Receptor for apelin coupled to G proteins that inhibit adenylate cyclase activity and plays a role in various processes in adults such as regulation of blood pressure, heart contractility, and heart failure. Also plays a key role in early development such as gastrulation and heart morphogenesis by acting as a receptor for APELA hormone. Alternative coreceptor with CD4 for HIV-1 infection; may be involved in the development of AIDS dementia.

Size: 100µL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Predicted Molecular Weight: 43


Cross Reactive Species: Mouse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Ustunel, Ismail, et al. "The effects of water immersion and restraint stress on the expressions of apelin, apelin receptor (APJR) and apoptosis rate in the rat heart." Acta Histochemica (2014)Read more>>
  • Izgüt-Uysal, V. N., et al. "The effect of apelin on the functions of peritoneal macrophages." Physiological research (2017).Read more>>
  • Lu Dinggui. et al. Apelin Alleviates Meniscus Endothelial Cell Apoptosis in Osteoarthritis. Dis Markers. 2022;2022:3556372Read more>>
  • Cemre Nur Balci. et al. The investigation of apelin and apelin receptor expressions in mouse endometrium during peri-implantation period. ANN ANAT. 2023 Feb;246:152027Read more>>
VALIDATION IMAGES

Mouse splenocytes probed with Rabbit Anti-APJ Receptor Polyclonal Antibody, Unconjugated (bs-2430R) at 1:100 for 30 minutes followed by incubation with a FITC conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).


Mouse heart lysates probed with Rabbit Anti-APJ Receptor Polyclonal Antibody, Unconjugated (bs-2430R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at RT.


Mouse cerebrum and heart lysates probed with APJ Receptor Polyclonal Antibody, unconjugated (bs-2430R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.


Human U251 cells probed with APJ Receptor Polyclonal Antibody, Unconjugated (bs-2430R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).