bs-2430R [Primary Antibody]
Apelin Receptor Polyclonal Antibody
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Host: Rabbit

Target Protein: Apelin Receptor

Immunogen Range: 52-66/380


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 187

Swiss Prot: P35414

Source: KLH conjugated synthetic peptide derived from human Apelin receptor (cytoplasmic domain)

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Receptor for apelin coupled to G proteins that inhibit adenylate cyclase activity and plays a role in various processes in adults such as regulation of blood pressure, heart contractility, and heart failure. Also plays a key role in early development such as gastrulation and heart morphogenesis by acting as a receptor for APELA hormone. Alternative coreceptor with CD4 for HIV-1 infection; may be involved in the development of AIDS dementia.

Size: 100µL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Predicted Molecular Weight: 43


Cross Reactive Species: Mouse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Ustunel, Ismail, et al. "The effects of water immersion and restraint stress on the expressions of apelin, apelin receptor (APJR) and apoptosis rate in the rat heart." Acta Histochemica (2014)Read more>>
  • Izgüt-Uysal, V. N., et al. "The effect of apelin on the functions of peritoneal macrophages." Physiological research (2017).Read more>>
  • Lu Dinggui. et al. Apelin Alleviates Meniscus Endothelial Cell Apoptosis in Osteoarthritis. Dis Markers. 2022;2022:3556372Read more>>
  • Cemre Nur Balci. et al. The investigation of apelin and apelin receptor expressions in mouse endometrium during peri-implantation period. ANN ANAT. 2023 Feb;246:152027Read more>>
VALIDATION IMAGES

Mouse heart lysates probed with Rabbit Anti-APJ Receptor Polyclonal Antibody, Unconjugated (bs-2430R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at RT.


Mouse splenocytes probed with Rabbit Anti-APJ Receptor Polyclonal Antibody, Unconjugated (bs-2430R) at 1:100 for 30 minutes followed by incubation with a FITC conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).


Mouse cerebrum and heart lysates probed with APJ Receptor Polyclonal Antibody, unconjugated (bs-2430R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.


Human U251 cells probed with APJ Receptor Polyclonal Antibody, Unconjugated (bs-2430R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).