bs-2440R [Primary Antibody]
IRAK4 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: IRAK4

Immunogen Range: 21-120/460


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 51135

Swiss Prot: Q9NWZ3

Source: KLH conjugated synthetic peptide derived from human IRAK4

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 51


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Cow
Sheep
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Cui Y et al. Loganin prevents BV‐2 microglia cells from Aβ1‐42‐induced inflammation via regulating TLR4/TRAF6/NF‐κB axis.(2019) Cell Biol Int.Read more>>
  • Wu Yutong. et al. Osteoclast-Derived Apoptotic Bodies Inhibit Naive Cd8<sup> </sup> T Cell Activation via Siglec15 Promoting Breast Cancer Secondary Metastasis. Cell Reports Medicine. 2022 Nov 03Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human endometrium carcinoma labeled with Rabbit Anti IRAK4 Polyclonal Antibody, Unconjugated (bs-2440R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Lane 1: Mouse Muscle lysates; Lane 2: Mouse Stomach lysates; Lane 3: Rat Muscle lysates probed with IRAK4 Polyclonal Antibody, Unconjugated (bs-2440R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.