bs-2441R-HRP [Conjugated Primary Antibody]
TWIST1/2 Polyclonal Antibody, HRP Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: TWIST1 and TWIST2

Specificity: This antibody will detect TWIST1 and TWIST2

Immunogen Range: 71-170/202


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7291; 117581

Swiss Prot: Q15672; Q8WVJ9

Source: KLH conjugated synthetic peptide derived from human TWIST1

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

TWIST1 and TWIST2 are basic helix-loop-helix transcriptional repressors that bind to E boxes in gene promoters, acting as master regulators in a variety of biological processes, including organogenesis, osteogenesis, cancer progression and hematopoietic cell development. Both TWIST1 and TWIST2 are found to act as Homodimers or Heterodimers with another bHLH protein. TWIST1 Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. TWIST1 also represses expression of proinflammatory cytokines such as TNFA and IL1B. TWIST2 inhibits transcriptional activation by MYOD1, MYOG, MEF2A, and MEF2C. Twist2 inhibits the premature or ectopic differentiation of preosteoblast cells during osteogenesis.

Conjugation: HRP

Excitation/ Emission: N/A

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)

Predicted Molecular Weight: 23


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhang, Lei, et al. "High-Throughput RNAi Screening Identifies a Role for the Osteopontin Pathway in Proliferation and Migration of Human Aortic Smooth Muscle Cells." Cardiovascular Drugs and Therapy (2016): 1-15.Read more>>
  • Xu, Shichen, et al. "Capsaicin inhibits metastasis of human papillary thyroid carcinoma BCPAP cells through modulation of the TRPV1 channel." Food & Function (2017).Read more>>
  • Wang et al. Kukoamine A inhibits human glioblastoma cell growth and migration through apoptosis induction and epithelial-mesenchymal transition attenuation. (2016) Sci.Rep. 6:36543Read more>>
  • Shen et al. Ube2v1-mediated ubiquitination and degradation of Sirt1 promotes metastasis of colorectal cancer by epigenetically suppressing autophagy. (2018) J.Hematol.Oncol. 11:95Read more>>
  • Ma T et al. MiR-940 inhibits migration and invasion of tongue squamous cell carcinoma via regulatingCXCR2/NF-κB system-mediated epithelial–mesenchymal transition. Naunyn Schmiedebergs Arch Pharmacol. 2019 Jun 18. Read more>>
  • Chang J et al. miR‐363‐3p inhibits migration, invasion, and epithelial–mesenchymal transition by targeting NEDD9 and SOX4 in non‐small‐cell lung cancer. J Cell Physiol. 2019 Jul 22. Read more>>
  • Junmin Li. et al. Circ_ZFR contributes to the paclitaxel resistance and progression of non-small cell lung cancer by upregulating KPNA4 through sponging miR-195-5p. Cancer Cell Int. 2021 Dec;21(1):1-15Read more>>
  • Jin, Meiyuan. et al. Role of ARID1A in the Regulation of Human Trophoblast Migration and Invasion. 2021 Jul 13Read more>>
  • Jian Shi. et al. FOXP4 promotes laryngeal squamous cell carcinoma progression through directly targeting LEF?1. Mol Med Rep. 2021 Dec;24(6):1-1Read more>>
  • Yonghe Li. et al. SOS1 regulates HCC cell epithelial-mesenchymal transition via the PI3K/AKT/mTOR pathway. BIOCHEM BIOPH RES CO. 2022 NovRead more>>
  • Haoming Chen. et al. Nicotine exposure exacerbates silica-induced pulmonary fibrosis via STAT3-BDNF-TrkB-mediated epithelial-mesenchymal transition in alveolar type II cells. FOOD CHEM TOXICOL. 2023 May;175:113694Read more>>
  • Xiaobei Zhang. et al. The roles and molecular mechanisms of long non-coding RNA WT1-AS in the maintenance and development of gastric cancer stem cells.. HELIYON. 2023 Mar;9(4):e14655-e14655Read more>>
  • Wang Wenjun. et al. LncRNA NEAT1 antagonizes the inhibition of melanoma proliferation, migration, invasion and EMT by Polyphyllin B. N-S ARCH PHARMACOL. 2023 Apr;:1-12Read more>>
  • E-Li Wang. et al. Cerebellin-2 promotes endothelial-mesenchymal transition in hypoxic pulmonary hypertension rats by activating NF-B/HIF-1/Twist1 pathway. LIFE SCI. 2023 Sep;328:121879Read more>>
  • Jinhua Gu. et al. Deciphering the mechanism of Peptostreptococcus anaerobius-induced chemoresistance in colorectal cancer: the important roles of MDSC recruitment and EMT activation. FRONT IMMUNOL. 2023; 14: 1230681Read more>>
  • Jie Chen. et al. Quercetin ameliorates liver fibrosis in Wilson disease and EMT involving suppression of the Hedgehog signaling pathway. ARAB J CHEM. 2024 Jan;17:105487Read more>>
  • Jin Linbo. et al. Early short-term hypoxia promotes epidermal cell migration by activating the CCL2-ERK1/2 pathway and epithelialmesenchymal transition during wound healing. BURNS TRAUMA. 2024 Jun;12Read more>>
VALIDATION IMAGES