bs-2442R [Primary Antibody]
MyoD1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: MyoD1

Immunogen Range: 85-130/318

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 17927

Swiss Prot: P10085

Source: KLH conjugated synthetic peptide derived from mouse MyoD1

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage Condition: Store at -20°C for 12 months.

Background: Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-1000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)
IF(ICC)(1:50-200)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Dai, Yang, et al. "microRNA-128 regulates the proliferation and differentiation of bovine skeletal muscle satellite cells by repressing Sp1." Molecular and Cellular Biochemistry (2016): 1-10.Read more>>
  • Shiratsuchi, Takaki, et al. "Sonic Hedgehog Supplementation Rapidly induces Myogenesis in Human Induced Pluripotent Stem Cells." Journal of St. Marianna University 6.2 (2015): 225-233.Read more>>
  • Sente, Tahnee, et al. "Primary skeletal muscle myoblasts from chronic heart failure patients exhibit loss of anti-inflammatory and proliferative activity."BMC Cardiovascular Disorders 16.1 (2016): 1.Read more>>
  • Kou YT et al. A transient protective effect of low-level laser irradiation against disuse-induced atrophy of rats. Lasers Med Sci. 2019 Apr 4.Read more>>
  • Dawson LE et al. Induction of Myogenic Differentiation Improves Chemosensitivity of Chemoresistant Cells in Soft-Tissue Sarcoma Cell Lines. Sarcoma. 2020 Mar 26;2020:8647981.Read more>>
  • Dawson LE et al. Induction of Myogenic Differentiation Improves Chemosensitivity of Chemoresistant Cells in Soft-Tissue Sarcoma Cell Lines. Sarcoma. 2020 Mar 26;2020:8647981.Read more>>
VALIDATION IMAGES

Lane 1: mouse muscle lysates; Lane 2: mouse heart lysates probed with MyoD1 Polyclonal Antibody, Unconjugated (bs-2442R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.


Formalin-fixed and paraffin embedded rat embryonic rhabdomyoma labeled with Anti-MyoD1/Myf3 Polyclonal Antibody, Unconjugated (bs-2442R) at 1:200 followed by conjugation to the secondary antibody


Lane 1: Rat muscle lysates; Lane 2: Mouse muscle lysates; Lane probed with MyoD1 Polyclonal Antibody, unconjugated (bs-2442R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.