bs-24447R [Primary Antibody]
HDAC1 Polyclonal Antibody
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Host: Rabbit

Target Protein: HDAC1

Immunogen Range: 1-100/480


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3065

Swiss Prot: Q13547

Source: KLH conjugated synthetic peptide derived from mouse HDAC1 (Nuclear Loading Control)

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B. Component a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development.

Size: 100uL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)

Predicted Molecular Weight: 53


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with HDAC1 polyclonal antibody, unconjugated (bs-24447R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.