bs-24507R [Primary Antibody]
C-Myc Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: C-Myc

Immunogen Range: 271-370/438


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4609

Swiss Prot: P01106

Source: KLH conjugated synthesised phosphopeptide derived from human C-Myc around the phosphorylation site of Ser373

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)

Predicted Molecular Weight: 49


Predicted Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Ling Xie. et al. Suppression of GOLM1 by EGCG through HGF/HGFR/AKT/GSK-3/-catenin/c-Myc signaling pathway inhibits cell migration of MDA-MB-231. Food Chem Toxicol. 2021 Nov;157:112574Read more>>
VALIDATION IMAGES

Lane 1: Human Raji cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human Jurkat cell lysates; Lane 4: Human K562 cell lysates ; Lane 5: Human Molt-4 cell lysates; Lane 6: Human U937 cell lysates; Lane 7: Human HL60 cell lysates; Lane 8: Human HepG2 cell lysates probed with C-Myc Polyclonal Antibody,Unconjugated (bs-24507R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.