bs-2458R [Primary Antibody]
IL4R Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: IL4R

Immunogen Range: 201-300/825

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3566

Swiss Prot: P24394

Source: KLH conjugated synthetic peptide derived from human IL4R

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage: Store at -20°C for 12 months..

Background: Receptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2. Soluble IL4R (sIL4R) inhibits IL4-mediated cell proliferation and IL5 up-regulation by T-cells.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-1000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 88

Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Danelli et al. Mast cells boost myeloid-derived suppressor cell activity and contribute to the development of tumor-favoring microenvironment. (2015) Cancer.Immunol.Res. 3:85-95Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti IL-4R/CD124 Plastin Polyclonal Antibody, Unconjugated (bs-2458R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded human colon carcinoma labeled Anti-IL-4R/CD124 Polyclonal Antibody, Unconjugated (bs-2458R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, AF488 conjugated(bs-0295G-AF488)used at 1:200 dilution


Human Raji Cells were fixed with 2% PFA (10min at room temperature). The cells were then incubated in 1 X PBS containing 0.5% BSA + 10% goat serum to block non-specific protein-protein interactions for 15 min at room temperature. Staining with IL4R Polyclonal Antibody, Unconjugated (bs-2458R) was performed at 1:100 for 30 min at room temperature. The secondary antibody, Goat anti-rabbit IgG-PE, was used at a 1:200 dilution for 40 min at room temperature. Primary antibody staining (green) is compared to compared to unstained cells (dark blue), secondary only (light blue), and isotype control (orange).