bs-24606R [Primary Antibody]
HIF 2 alpha Polyclonal Antibody
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Host: Rabbit

Target Protein: HIF 2 alpha

Immunogen Range: 471-570/870


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2034

Swiss Prot: Q99814

Source: KLH conjugated synthetic peptide derived from human HIF 2 alpha

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD.

Size: 100uL

Concentration: 1ug/ul

Applications: IHC-P(1:400-800)
IHC-F(1:400-800)
IF(1:100-500)

Predicted Molecular Weight: 96


Cross Reactive Species: Rat

Predicted Cross Reactive Species: Human
Pig

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HIF 2 alpha Polyclonal Antibody, Unconjugated (bs-24606R) at 1:200 overnight at 4°C, DAB staining.