bs-2469R
[Primary Antibody]
PERK Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: PERK
Immunogen Range: 1001-1116/1116
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 9451
Swiss Prot: Q9NZJ5
Source: KLH conjugated synthetic peptide derived from human PER
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function (By similarity).
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 122
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Wang, Yu, et al. "Tanshinone II A Relieves Adriamycin-induced Myocardial Injury in Rat Model." International Journal of Chemistry 8.1 (2016): 40.Read more>>
- Yu, H., et al. "Gypenoside Protects against Myocardial Ischemia-Reperfusion Injury by Inhibiting Cardiomyocytes Apoptosis via Inhibition of CHOP Pathway and Activation of PI3K/Akt Pathway In Vivo and In Vitro."Cellular Physiology and Biochemistry 39.1 (2016): 123-136.Read more>>
- Yan, Jiting, et al. "Catalpol prevents alteration of cholesterol homeostasis in non-alcoholic fatty liver disease via attenuating endoplasmic reticulum stress and NOX4 over-expression." RSC Advances 7.2 (2017): 1161-1176.Read more>>
- Li et al. Apoptosis Induction by Iron Radiation via Inhibition of Autophagy in Trp53+/- Mouse Testes: Is Chronic Restraint-Induced Stress a Modifying Factor?. (2018) Int.J.Biol.Sci. 14:1109-1121Read more>>
- Li,et al.Silver nanoparticles induce SH-SY5Y cell apoptosis via endoplasmic reticulum- and mitochondrial pathways that lengthen endoplasmic reticulum-mitochondria contact sites and alter inositol-3-phosphate receptor function.(2018) Toxicology Letters. 285:156-167.Read more>>
- Duan,et al.Exposure to DBP and High Iodine Aggravates Autoimmune Thyroid Disease Through Increasing the Levels of IL-17 and Thyroid-Binding Globulin in Wistar Rats.(2018) Toxicological Sciences. 163:196-205.Read more>>
- Qi,et al.Cytosolic β-glucosidase inhibition and renal blood flow suppression are leading causes for the enhanced systemic exposure of salidroside in hypoxic rats.(2018) RSC Advances. 8:8469-8483.Read more>>
- Wang L et al. Radioprotective effect of Hohenbuehelia serotina polysaccharides through mediation of ER apoptosis pathway in vivo. Int J Biol Macromol. 2019 Apr 15;127:18-26. Read more>>
- Cui J et al. Acetaldehyde Induces Neurotoxicity In Vitro via Oxidative Stress-and Ca2. Oxid Med Cell Longev. 2019 Jan 9;2019:2593742. Read more>>
- Wenfeng Gouet al. Ursolic acid derivative UA232 evokes apoptosis of lung cancer cells induced by endoplasmic reticulum stress. Pharm Biol. 2020 Dec;58(1):707-715.Read more>>
- Rui Ding. et al. Endoplasmic reticulum stress and oxidative stress contribute to neuronal pyroptosis caused by cerebral venous sinus thrombosis in rats: Involvement of TXNIP/peroxynitrite-NLRP3 inflammasome activation. Neurochem Int. 2020 Dec;141:104856Read more>>
- Yujie Zhong. et al. Inhibition of ER stress attenuates kidney injury and apoptosis induced by 3-MCPD via regulating mitochondrial fission/fusion and Ca 2+ homeostasis. 2021 Mar 02Read more>>
- Yujie Zhong. et al. Jujuboside A ameliorates high fat diet and streptozotocin induced diabetic nephropathy via suppressing oxidative stress, apoptosis, and enhancing autophagy. Food Chem Toxicol. 2022 Jan;159:112697Read more>>
- Yujie Zhong. et al. Dioscin relieves diabetic nephropathy via suppressing oxidative stress, apoptosis, and improving mitochondrial quality and quantity control. Food Funct. 2022 FebRead more>>
- Li Wang. et al. New sight into interaction between endoplasmic reticulum stress and autophagy induced by vanadium in duck renal tubule epithelial cells. CHEM-BIOL INTERACT. 2022 May;:109981Read more>>
- Changzheng Li. et al. Amino acid catabolism regulates hematopoietic stem cell proteostasis via a GCN2-eIF2_ axis. CELL STEM CELL. 2022 Jul;29:1119Read more>>
- Xue Li. et al. Dual regulation on oxidative stress and endoplasmic reticulum stress by [70] fullerenes for reversing insulin resistance in diabetes. NANO TODAY. 2022 Aug;45:101541Read more>>
- Shuai Jiang. et al. Cardiac-specific overexpression of Claudin-5 exerts protection against myocardial ischemia and reperfusion injury. BBA-MOL BASIS DIS. 2022 Sep;:166535Read more>>
- Yujie Zhong. et al. Diosgenin Ameliorated Type II Diabetes-Associated Nonalcoholic Fatty Liver Disease through Inhibiting De Novo Lipogenesis and Improving Fatty Acid Oxidation and Mitochondrial Function in Rats. NUTRIENTS. 2022 Jan;14(23):4994Read more>>
- Metin, Tuba Ozcan. et al. Expression of ER stress markers (GRP78 and PERK) in experimental nephrotoxicity induced by cisplatin and gentamicin: roles of inflammatory response and oxidative stress. N-S ARCH PHARMACOL. 2022 Dec;:1-13Read more>>
- Eun-Hye Seo. et al. The Effect of Ketamine on Endoplasmic Reticulum Stress in Rats with Neuropathic Pain. INT J MOL SCI. 2023 Jan;24(6):5336Read more>>
- Yujie Zhong. et al. Diosgenin Inhibits ROS Generation by Modulating NOX4 and Mitochondrial Respiratory Chain and Suppresses Apoptosis in Diabetic Nephropathy. NUTRIENTS. 2023 Jan;15(9):2164Read more>>
- Jinlong Tan. et al. Emerging evidence for poxvirus-mediated unfolded protein response: Lumpy skin disease virus maintains self-replication by activating PERK and IRE1 signaling. FASEB J. 2023 Apr;37(5):e22902Read more>>
- Dandan Wu. et al. Unfolded Protein Response Factor ATF6 Augments T Helper Cell Responses and Promotes Mixed Granulocytic Airway Inflammation. MUCOSAL IMMUNOL. 2023 MayRead more>>
- Yulin Zhang. et al. Protective effect of bioactive components from Rubi fructus against oxidative damage in human ovarian granulosa cells induced by 2,2-azobis (2-methylpropionamidine) dihydrochloride. J SCI FOOD AGR. 2024 FebRead more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded mouse stomach labeled with Anti-PERK Polyclonal Antibody, Unconjugated (bs-2469R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n
Paraformaldehyde-fixed, paraffin embedded rat kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-PERK Polyclonal Antibody, Unconjugated (bs-2469R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining
U-87MG cells were fixed with 4% PFA for 10min at room temperature ，permeabilized with 20% PBST for 20 min at room temperature , and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PERK Polyclonal Antibody at 1：50 dilution in blocking buffer and incubated for 30 min at -20℃, washed twice with 2%BSA in PBS, followed bysecondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed.
Lane 1: Hela cell lysates; Lane 2: 293T cell lysates probed with PERK Polyclonal Antibody, Unconjugated (bs-2469R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Cerebrum lysates; Lane 2: Rat Cerebrum lysates probed with PERK Polyclonal Antibody, Unconjugated (bs-2469R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PERK Polyclonal Antibody, Unconjugated (bs-2469R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PERK Polyclonal Antibody, Unconjugated (bs-2469R) at 1:200 overnight at 4°C, DAB staining.
K562 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PERK Polyclonal Antibody(bs-2469R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).