Host: Rabbit
Target Protein: MBP
Immunogen Range: 241-304/304
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 4155
Swiss Prot: P02686
Source: KLH conjugated synthetic peptide derived from human MBP
Purification: affinity Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1mg/ml
Applications:
WB(WB=1:500-2000)
IHC-P(1:500-2000)
IHC-F(1:500-2000)
IF(1:500-2000)
Predicted Molecular Weight: 33
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Sheep
Pig
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
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Lane 1: Human HL60 cell lysates; Lane 2: Human U937 cell lysates; Lane 3: Human U87MG cell lysates probed with MBP Polyclonal Antibody, Unconjugated (bs-24827R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (bs-24827R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (bs-24827R) at 1:200 overnight at 4°C, DAB staining.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebrum tissue lysates; Lane 3: Rat Cerebellum tissue lysates probed with MBP Polyclonal Antibody, Unconjugated (bs-24827R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MBP Polyclonal Antibody, Unconjugated (bs-24827R) at 1:1500 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MBP Polyclonal Antibody, Unconjugated (bs-24827R) at 1:1500 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MBP Polyclonal Antibody, Unconjugated (bs-24827R) at 1:1500 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MBP Polyclonal Antibody, Unconjugated (bs-24827R) at 1:1500 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.