bs-25013R [Primary Antibody]
ATG5/APG5L Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ATG5/APG5L

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 9474

Swiss Prot: Q9H1Y0

Source: KLH conjugated synthetic peptide derived from human ATG5/APG5L

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (By similarity). May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)

Predicted Molecular Weight: 65


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Dog
Cow
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Qiuxiang Cai. et al. Pasteurella multocida causes liver injury in ducks by mediating inflammatory, apoptotic and autophagic pathways. MICROB PATHOGENESIS. 2023 Sep;:106336Read more>>
  • ohan Chen. et al. Inhalation of ammonia promotes apoptosis and induces autophagy in hepatocytes via Bax/BCl-2 and m-TOR/ATG5/LC-3bII axes. SCI TOTAL ENVIRON. 2024 Feb;912:169036Read more>>
VALIDATION IMAGES

Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Stomach tissue lysates; Lane 3: Rat Cerebrum tissue lysates probed with ATG5/APG5L Polyclonal Antibody, Unconjugated (bs-25013R) at 1:300 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Human PANC-1 cell lysates; Lane 2: Human U87MG cell lysates probed with ATG5/APG5L Polyclonal Antibody, Unconjugated (bs-25013R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.