bs-2501R [Primary Antibody]
KiSS1 receptor Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: KiSS1 receptor

Immunogen Range: 121-200/398


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 84634

Swiss Prot: Q969F8

Source: KLH conjugated synthetic peptide derived from human KiSS1 receptor

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Receptor for metastin (kisspeptin-54 or kp-54), a C-terminally amidated peptide of KiSS1. KiSS1 is a metastasis suppressor protein that suppresses metastases in malignant melanomas and in some breast carcinomas without affecting tumorigenicity. The metastasis suppressor properties may be mediated in part by cell cycle arrest and induction of apoptosis in malignant cells. The receptor is essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/KISS1R system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood. The receptor is also probably involved in the regulation and fine-tuning of trophoblast invasion generated by the trophoblast itself. Analysis of the transduction pathways activated by the receptor identifies coupling to phospholipase C and intracellular calcium release through pertussis toxin-insensitive G(q) proteins.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)

Predicted Molecular Weight: 44


Cross Reactive Species: Human
Mouse
Rat
Others

Predicted Cross Reactive Species: Dog
Sheep
Pig
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Luo, Qianqian, et al. "Leptin/leptinR-kisspeptin/kiss1r-GnRH pathway reacting to regulate puberty onset during negative energy balance." Life Sciences 153 (2016): 207-212.Read more>>
  • Chaikhun-Marcou, T. "Localization of Kisspeptin Receptors in Buffalo Heifer Pituitary Glands."Read more>>
  • Luo, Qian-Qian, et al. "The role of kisspeptin/kiss1r system expressed in the arcuate nucleus of hypothalamus on the development and maintenance of reproduction during developing female rats." Journal of the Chinese Medical Association (2016).Read more>>
  • Chaikhun-Marcou, Thuchadaporn, et al. "Evidence of kisspeptin receptor expression in GnRH neurons in the preoptic area and arcuate hypothalamic nuclei in cycling buffaloes." The Thai Journal of Veterinary Medicine 46.3 (2016): 381-390.Read more>>
  • Liu et al. Di-(2-ethylhexyl) phthalate induces precocious puberty in adolescent female rats. (2018) Iran.J.Basic.Med.Sci. 21:848-855Read more>>
  • Jin H et al. Microcystin-leucine arginine induced the apoptosis of GnRH neurons by activating the endoplasmic reticulum stress resulting in a decrease of serum testosterone level in miceEcotoxicol Environ Saf.2021 Jan 15;208:111748.Read more>>
  • Annamria Schaffer. et al. The ontogeny of kisspeptin receptor in the uterine contractions in rats: Its possible role in the quiescence of non-pregnant and pregnant uteri. Eur J Pharmacol. 2021 Apr;896:173924Read more>>
  • Junpeng Feng. et al. The effect of high-fat diet and exercise on KISS-1/GPR54 expression in testis of growing rats. Nutr Metab. 2021 Dec;18(1):1-1Read more>>
  • Yi Huang. et al. Kisspeptin-54 attenuates oxidative stress and neuronal apoptosis in early brain injury after subarachnoid hemorrhage in rats via GPR54/ARRB2/AKT/GSK3 signaling pathway. Free Radical Bio Med. 2021 Aug;171:99Read more>>
  • Elena Mele. et al. Differential Expression of Kisspeptin System and Kisspeptin Receptor Trafficking during Spermatozoa Transit in the Epididymis. Genes-Basel. 2022 Feb;13(2):295Read more>>
  • Lu Xiaosheng. et al. Deficiency of C1QL1 reduced murine ovarian follicle reserve through intraovarian and endocrine control. ENDOCRINOLOGY. 2022 ApRead more>>
  • Luz Patricia Muoz de la Torre. et al. Sex-specific hypothalamic expression of kisspeptin, gonadotropin releasing hormone, and kisspeptin receptor in progressive demyelination model. J CHEM NEUROANAT. 2022 Sep;123:10212Read more>>
VALIDATION IMAGES

Lane 1: Mouse Spinal cord lysates; Lane 2: Rat Spinal cord lysates probed with KiSS1 receptor Polyclonal Antibody, Unconjugated (bs-2501R) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.