bs-2503R-A647 [Conjugated Primary Antibody]
eEF1A1 Polyclonal Antibody, ALEXA FLUOR® 647 Conjugated
www.biossusa.com
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DATASHEET

Host: Rabbit

Target Protein: eEF1A1

Specificity: May crossreact with EEF1A1P5 due to high sequence similarity.

Immunogen Range: 131-230/462


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1915

Swiss Prot: P68104

Source: KLH conjugated synthetic peptide derived from human eEF1A1

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. With PARP1 and TXK, forms a complex that acts as a T helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.

Conjugation: ALEXA FLUOR® 647

Excitation/ Emission: 650nm/665nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 50


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Pig
Horse
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Oh et al. Analysis of macrophage phenotype in rejected corneal allografts. (2013) Invest.Ophthalmol.Vis.Sc. 54:7779-84Read more>>
  • Zhang M et al. Proteomic analysis reveals proteins and pathways associated with lactation in bovine mammary epithelial cell-derived exosomes. J Proteome Res. 2020 Aug 7;19(8):3211-3219.Read more>>
  • Lin Zhu. et al. The MERS-CoV N Protein Regulates Host Cytokinesis and Protein Translation via Interaction With EF1A. Front Microbiol. 2021; 12: 551602Read more>>
  • Martin Schicht. et al. Ocular surface changes in mice with streptozotocin-induced diabetes and diabetic polyneuropathy. OCUL SURF. 2023 DecRead more>>