Host: Rabbit
Target Protein: CD44
Immunogen Range: 325-429/429
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 960
Swiss Prot: P16070
Source: KLH conjugated synthetic peptide derived from human CD44
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 53
Cross Reactive Species:
Human
Mouse
Rat
Chicken
Predicted Cross Reactive Species:
Cow
Pig
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
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Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CD44 Polyclonal Antibody, Unconjugated (bs-2507R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded chicken ileum labeled with Anti-CD44V6 Polyclonal Antibody, Unconjugated (bs-2507R) at 1:300 followed by conjugation to the secondary antibody
Hela cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD44 Polyclonal Antibody(bs-2507R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).