bs-2511R [Primary Antibody]
Acetylcholinesterase Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Acetylcholinesterase

Immunogen Range: 521-614/614


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 43

Swiss Prot: P22303

Source: KLH conjugated synthetic peptide derived from human AchE

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 68


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Dog
Cow
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Shao, Yi, et al. "Inhibition of miR-134 Protects Against Hydrogen Peroxide-Induced Apoptosis in Retinal Ganglion Cells." Journal of Molecular Neuroscience (2015): 1-11.Read more>>
  • Roch, Aline, Vincent Trachsel, and Matthias P. Lutolf. "Single cell analysis reveals cell division‐independent emergence of megakaryocytes from phenotypic hematopoietic stem cells." STEM CELLS (2015).Read more>>
  • Wang, Jianlong, et al. "Basic fibroblast growth factor attenuates the degeneration of injured spinal cord motor endplates." Neural Regeneration Research 8.24 (2013): 2213.Read more>>
  • Wei Lu. et al. Molecular mechanisms by which targeted muscle reinnervation improves the microenvironment of spinal cord motor neurons and target muscles. NEUROSCI LETT. 2022 Oct;789:136879Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat heart labeled with Anti- ACHE/Acetylcholinesterase Polyclonal Antibody, Unconjugated (bs-2511R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3) used at 1:200 dilution for 40 minutes at 37°C


Lane 1: Human K562 cell lysates; Lane 2: Human U-2 OS cell lysates; Lane 3: Human MCF-7 cell lysates; Lane 4: Human SH-SY5Y cell lysates probed with ACHE Polyclonal Antibody, Unconjugated (bs-2511R) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.