bs-2513R
[Primary Antibody]
NFKBIA(Ser32/36) Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: NFKBIA Ser32/36
Specificity: These phosphorylation sites are homologous among the species listed.
Modification Site: Ser32/36
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 4792
Swiss Prot: P25963
Source: KLH conjugated synthetic phosphopeptide derived from human IKB alpha around the phosphorylation site of Ser32/36
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 35
Cross Reactive Species:
Human
Mouse
Rat
Chicken
Predicted Cross Reactive Species:
Dog
Cow
Sheep
Pig
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Fu, Juanli, et al. "Tetrachlorobenzoquinone exhibits neurotoxicity by inducing inflammatory responses through ROS-mediated IKK/IκB/NF-κB signaling."Environmental Toxicology and Pharmacology (2016).Read more>>
- Li, Xue-Nan, et al. "Lycopene mitigates atrazine-induced cardiac inflammation via blocking the NF-κB pathway and NO production." Journal of Functional Foods 29 (2017): 208-216.Read more>>
- Gong et al. Rho Kinase Type 1 (ROCK1) Promotes Lipopolysaccharide-induced Inflammation in Corneal Epithelial Cells by Activating Toll-Like Receptor 4 (TLR4)-Mediated Signaling. (2018) Med.Sci.Monit. 24:3514-3523Read more>>
- Li Y et al. Silencing of LncRNA Steroid Receptor RNA Activator attenuates polycystic ovary syndrome in Mice. (2018)Biochimie; Nov 1;157:48-56.Read more>>
- Dai M et al.Pravastatin sodium attenuated TREM-1-mediated inflammation in human peripheral blood mononuclear cells.(2019)Biochem Biophys Res Commun. Jan 1;508(1):225-229.Commun.Read more>>
- Lv R et al. Polydatin alleviates traumatic spinal cord injury by reducing microglial inflammation via regulation of iNOS and NLRP3 inflammasome pathway. Int Immunopharmacol. 2019 Feb 18;70:28-36.Read more>>
- Yan B et al. Suppression of puerarin on polymethylmethacrylate-induced lesion of peri-implant by inhibiting NF-κB activation in vitro and in vivo.Pathol Res Pract. 2019 Mar 1:152372. Read more>>
- Ma T et al. MiR-940 inhibits migration and invasion of tongue squamous cell carcinoma via regulatingCXCR2/NF-κB system-mediated epithelial–mesenchymal transition. Naunyn Schmiedebergs Arch Pharmacol. 2019 Jun 18. Read more>>
- Xin H et al. miR-196a-5p promotes metastasis of colorectal cancer via targeting IκBα. BMC Cancer. 2019 Jan 8;19(1):30. Read more>>
- Fang D et al. Overexpression of Biglycan is Associated with Resistance to Rapamycin in Human WERI-Rb-1 Retinoblastoma Cells by Inducing the Activation of the Phosphatidylinositol 3-Kinases (PI3K)/Akt/Nuclear Factor kappa B (NF-κB) Signaling Pathway. Med Sci Monit. 2019 Sep 4;25:6639-6648. Read more>>
- Wu ZY et al. Baicalin Attenuates Mycoplasma gallisepticum-Induced Inflammation via Inhibition of the TLR2-NF-κB Pathway in Chicken and DF-1 Cells. Infection and Drug Resistance 2019:12 3911–3923.Read more>>
- Peng L et al. Madecassoside Protects Against LPS-Induced Acute Lung Injury via Inhibiting TLR4/NF-B Activation and Blood-Air Barrier Permeability. Front Pharmacol. 2020 Jun 5;11:807.Read more>>
- Feng Gao. et al. Aucubin ameliorates the LPS\induced inflammatory response in bovine endometrial epithelial cells by inhibiting NF\B and activating the Keap1/Nrf2 signalling pathway. 2021 Apr 27Read more>>
- Gaolong Zhong. et al. Arsenic exposure induces intestinal barrier damage and consequent activation of gut-liver axis leading to inflammation and pyroptosis of liver in ducks. Sci Total Environ. 2021 Sep;788:14778Read more>>
- Qihe Tang. et al. Bergenin Monohydrate Attenuates Inflammatory Response via MAPK and NF-B Pathways Against Klebsiella pneumonia Infection. Front Pharmacol. 2021; 12: 651664Read more>>
- Qingyu Meng. et al. Morin hydrate inhibits atherosclerosis and LPS-induced endothelial cells inflammatory responses by modulating the NFB signaling-mediated autophagy. Int Immunopharmacol. 2021 Nov;100:108096Read more>>
- Muhammad Ishfaq. et al. Baicalin alleviates Mycoplasma gallisepticum-induced oxidative stress and inflammation via modulating NLRP3 inflammasome-autophagy pathway. Int Immunopharmacol. 2021 Dec;101:10825Read more>>
- Yusong Miao. et al. Methylsulfonylmethane ameliorates inflammation via NF-_B and ERK/JNK-MAPK signaling pathway in chicken trachea and HD11 cells during Mycoplasma gallisepticum infection. Poultry Sci. 2022 Jan;:101706Read more>>
- Fang Wan. et al. Curcumin activates the Nrf2 Pathway to alleviate AFB1-induced immunosuppression in the spleen of ducklings. Toxicon. 2022 Apr;209:18Read more>>
- Liu Jiawei. et al. Lactobacillus rhamnosus GR-1 Alleviates Escherichia coli-Induced Inflammation via NF-_B and MAPKs Signaling in Bovine Endometrial Epithelial Cells. Front Cell Infect Mi. 2022 Mar;0:199Read more>>
- Jie Song. et al. Ethanol Extract of Artemisia Annua Prevents LPS-Induced Inflammation and Blood–Milk Barrier Disruption in Bovine Mammary Epithelial Cells. ANIMALS. 2022 Jan;12(10):1228Read more>>
- Zhang, Di. et al. Salvia miltiorrhiza polysaccharides ameliorates Staphylococcus aureus-induced mastitis in rats by inhibiting activation of the NF-_B and MAPK signaling pathways. BMC VET RES. 2022 Dec;18(1):1-11Read more>>
- Tang, Lixuan. et al. Curcumin antagonizes inflammation and autophagy induced by arsenic trioxide through immune protection in duck spleen. ENVIRON SCI POLLUT R. 2022 Jun;:1-12Read more>>
- Chenxue Mei. et al. CD30L is involved in the regulation of the inflammatory response through inducing homing and differentiation of monocytes via CCL2/CCR2 axis and NF-_B pathway in mice with colitis. INT IMMUNOPHARMACOL. 2022 Sep;110:108934Read more>>
- Rui Guo. et al. Ulinastatin attenuates spinal cord injury by targeting AMPK/NLRP3 signaling pathway. J CHEM NEUROANAT. 2022 Nov;125:102145Read more>>
- Li, Manman. et al. Anti-inflammation of isoliquiritigenin via the inhibition of NF-B and MAPK in LPS-stimulated MAC-T cells. BMC VET RES. 2022 Dec;18(1):1-1Read more>>
- Ya-Nan Gao. et al. Aflatoxin M1 and ochratoxin A induce a competitive endogenous RNA regulatory network of intestinal immunosuppression by whole-transcriptome analysis. SCI TOTAL ENVIRON. 2022 Sep;:158777Read more>>
- Yeke Wu. et al. Aconiti lateralis radix praeparata total alkaloids exert anti-RA effects by regulating NF-B and JAK/STAT signaling pathways and promoting apoptosis.. FRONT PHARMACOL. 2022 Sep;13:980229-980229Read more>>
- Xiaoyu Wang. et al. Two kinds of traditional Chinese medicine prescriptions reduce thymic inflammation levels and improve humoral immunity of finishing pigs.. FRONT VET SCI. 2022 Sep;9:929112-929112Read more>>
- Shikuo Rong. et al. Amentoflavone Exerts Anti-Neuroinflammatory Effects by Inhibiting TLR4/MyD88/NF-<i></i>B and Activating Nrf2/HO-1 Pathway in Lipopolysaccharide-Induced BV2 Microglia. MEDIAT INFLAMM. 2022 Dec 06;2022:5184721Read more>>
- Gao Jie-Fang. et al. Myricetin treatment has ameliorative effects in DNFB-induced atopic dermatitis mice under high-fat conditions. TOXICOL SCI. 2022 DecRead more>>
- Zhang Xiangjun. et al. DAD3 targets ACE2 to inhibit the MAPK and NF-B signalling pathways and protect against LPS-induced inflammation in bovine mammary epithelial cells. VET RES. 2022 Dec;53(1):1-13Read more>>
- Jianhao Yang. et al. Serum amyloid A regulates TLR2/4-mediated IFN- signaling pathway against Marek's disease virus. VIRUS RES. 2023 Jan;:199044Read more>>
- Li, Ying. et al. Parthenolide inhibits proliferation of cells infected with Kaposis sarcoma-associated herpesvirus by suppression of the NF-B signaling pathway. ARCH VIROL. 2023 Feb;168(2):1-12Read more>>
- Yuanyuan Xing. et al. Artemisia ordosica polysaccharide ameliorated LPS-induced growth inhibition and intestinal injury in broilers through enhancing immune-regulation and antioxidant capacity. J NUTR BIOCHEM. 2023 Feb;:109284Read more>>
- Zhen Xu. et al. Green Biosynthesis of Silver Nanoparticles Using Aqueous Extracts of Ageratum Conyzoides and Their Anti-Inflammatory Effects. ACS APPL MATER INTER. 2023;XXXX(XXX):XXX-XXXRead more>>
- Zhen Xu. et al. The aqueous extracts of Ageratum conyzoides inhibit inflammation by suppressing NLRP3 inflammasome activation. J ETHNOPHARMACOL. 2023 Jun;309:116353Read more>>
- Jing Fan. et al. Syndecan-3 Coregulates Milk Fat Metabolism and Inflammatory Reactions in Bovine Mammary Epithelial Cells through AMPK/SIRT1 Signaling Pathway. INT J MOL SCI. 2023 Jan;24(7):6657Read more>>
- Yani He. et al. Glycolytic reprogramming controls periodontitis-associated macrophage pyroptosis via AMPK/SIRT1/NF-B signaling pathway. INT IMMUNOPHARMACOL. 2023 Jun;119:110192Read more>>
- Jing Mao. et al. Sea Cucumber Hydrolysate Alleviates Immunosuppression and Gut Microbiota Imbalance Induced by Cyclophosphamide in Balb/c Mice through the NF-κB Pathway. FOODS. 2023 Jan;12(8):1604Read more>>
- Cheng Wang. et al. Inhibitions inflammatory response in clicks alleviates LPS induced myocardial injury by total polysaccharides of Pinus massoniana Lamb. pollen. Carbohydrate Polymer Technologies and Applications. 2023 Dec;6:100372Read more>>
- Lemiao Zhong. et al. Mycoplasma synoviae induce spleen tissue damage and inflammatory response of chicken through oxidative stress and apoptosis. VIRULENCE. 2023 Nov 16Read more>>
- Xin-Ran Ge. et al. Phthalate drives splenic inflammatory response via activating HSP60/TLR4/NLRP3 signaling axis-dependent pyroptosis. ENVIRON POLLUT. 2024 Apr;346:12361Read more>>
- Haipeng Feng. et al. Baicalin Protects Broilers against Avian Coronavirus Infection via Regulating Respiratory Tract Microbiota and Amino Acid Metabolism. INT J MOL SCI. 2024 Jan;25(4):2109Read more>>
- Jie Feng. et al. IL-35 ameliorates lipopolysaccharide-induced endothelial dysfunction by inhibiting endothelial-to-mesenchymal transition. INT IMMUNOPHARMACOL. 2024 Mar;129:111567Read more>>
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NFKBIA(Ser32/36) Polyclonal Antibody, Unconjugated (bs-2513R) at 1:200 overnight at 4°C, DAB staining.
Lane 1: Human HepG2 cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human Siha cell lysates; Lane 4: Human U251 cell lysates probed with NFKBIA Polyclonal Antibody, Unconjugated (bs-2513R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (phospho-IKB alpha (Ser32 + Ser36)) polyclonal Antibody, Unconjugated (bs-2513R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.