bs-2549R [Primary Antibody]
CXCL5 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CXCL5

Immunogen Range: 51-132/132


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 20311

Swiss Prot: P50228

Source: KLH conjugated synthetic peptide derived from mouse CXCL5

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

May participate in the recruitment of inflammatory cells by injured or infected tissue. GCP-2(1-78) and, more potent, GCP-2(9-78) attract neutrophils and are involved in neutrophil activation.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 14


Cross Reactive Species: Mouse
Rat
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Heilmann, Aline, et al. ʺThe Wnt Serpentine Receptor Frizzled-9 Regulates New Bone Formation in Fracture Healing.ʺ PloS one 8.12 (2013): e84232.Read more>>
  • DAlessio, Franco R., et al. "Lung Angiogenesis Requires CD4+ Foxp3+ Regulatory T Cells." American Journal of Respiratory Cell and Molecular Biology (2014)Read more>>
  • Gaballah, Mohammed Hassan, et al. "Simultaneous time course analysis of multiple markers based on DNA microarray in incised wound in skeletal muscle for wound aging." Forensic Science International (2016).Read more>>
  • Randall-Demllo, Sarron, et al. "Characterisation of colonic dysplasia-like epithelial atypia in murine colitis." World J Gastroenterol 22.37 (2016): 8334-8348.Read more>>
  • Ito, Ryoji, et al. "A novel xenogeneic graft‐versus‐host disease model for investigating the pathological role of human CD4+ or CD8+ T cells using immunodeficient NOG mice." American Journal of Transplantation (2016).Read more>>
  • Bortell et al. Astrocyte-specific overexpressed gene signatures in response to methamphetamine exposure in vitro. (2017) J.Neuroinflammatio. 14:49Read more>>
  • Wang et al. Targeting YAP-Dependent MDSC Infiltration Impairs Tumor Progression. (2016) Cancer.Disco. 6:80-95Read more>>
  • Jiang et al. Detection of Atherosclerotic Plaques in the Rabbit Aorta Using Ultrasound Microbubbles Conjugated to Interleukin-18 Antibodies. (2017) Med.Sci.Moni. 23:5446-5454Read more>>
  • Chinatsu Nishida. et al. Predictive Biomarkers for the Ranking of Pulmonary Toxicity of Nanomaterials. Nanomaterials-Basel. 2020 Oct;10(10):2032Read more>>
  • Ting-Ting Chang. et al. Inhibition on CXCL5 reduces aortic matrix metalloproteinase 9 expression and protects against acute aortic dissection. Vasc Pharmacol. 2021 Oct;:106926Read more>>
  • Nishida, Chinatsu. et al. Inflammogenic effect of polyacrylic acid in rat lung following intratracheal instillation. Part Fibre Toxicol. 2022 Dec;19(1):1-17Read more>>
  • Xin Ge. et al. Epigenetic alterations of CXCL5 in Cr(VI)-induced carcinogenesis. SCI TOTAL ENVIRON. 2022 Jun;:155713Read more>>
  • Yasuyuki Higashi. et al. Pulmonary disorder induced by cross-linked polyacrylic acid. J OCCUP HEALTH. 2022 Dec;64(1):e12369Read more>>
  • Qicong Luo. et al. Apatinib remodels the immunosuppressive tumor ecosystem of gastric cancer enhancing anti-PD-1 immunotherapy. CELL REP. 2023 Apr 24;42(5):112437Read more>>
  • Solangy Lizcano-Meneses. et al. Combined Inhibition of Indolamine-2,3-Dioxygenase 1 and C-X-C Chemokine Receptor Type 2 Exerts Antitumor Effects in a Preclinical Model of Cervical Cancer. BIOMEDICINES. 2023 Aug;11(8):228Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin-embedded Rat Brain tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20 minutes; Antibody incubation with CXCL5 Polyclonal Antibody, Unconjugated (bs-2549R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody and DAB staining.