bs-2558R [Primary Antibody]
EGFRv3 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: EGFRv3

Immunogen Range: 11-120/1081


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1956

Swiss Prot: Q59FL8

Source: KLH conjugated synthetic peptide derived from human EGFRvIII

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. Isoform 2 may act as an antagonist of EGF action.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 100/130


Cross Reactive Species: Human
Mouse
Chicken
Others

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wang, Jiansheng. "Clinical significance of EGFR and EGFRvIII expression in human esophageal carcinoma." Pakistan Journal of Medical Sciences 27.3 (2011).Read more>>
  • Wehrenberg-Klee, Eric, et al. "PET imaging of glioblastoma multiforme EGFR expression for therapeutic decision guidance." Am J Nucl Med Mol Imaging 5.4 (2015): 379-389.Read more>>
  • Wei, Jian-wei, et al. "F25P preproinsulin abrogates the secretion of pro-growth factors from EGFRvIII cells and suppresses tumor growth in an EGFRvIII/wt heterogenic model." Cancer Letters (2016).Read more>>
  • Agnes, Richard S., et al. "An optical probe for noninvasive molecular imaging of orthotopic brain tumors overexpressing epidermal growth factor receptor." Molecular cancer therapeutics 11.10 (2012): 2202-2211.Read more>>
  • Basilion, James, and Richard Agnes. "Targeted non-invasive imaging probes of egfr expressing cells." U.S. Patent Application No. 13/671,957.Read more>>
  • Kang, Chun-Sheng, et al. "EGFL7 is an intercellular EGFR signal messenger that plays an oncogenic role in glioma." Cancer Letters (2016).Read more>>
  • Liu et al. EGFRvIII/integrin β3 interaction in hypoxic and vitronectinenriching microenvironment promote GBM progression and metastasis. (2016) Oncotarge. 7:4680-94Read more>>
  • Khelwatty et al. The impact of co-expression of wild-type EGFR and its ligands determined by immunohistochemistry for response to treatment with cetuximab in patients with metastatic colorectal cancer. (2017) Oncotarget. 8:7666-7677Read more>>
  • Vettath,et al.Recombinant expression of extracellular domain of mutant Epidermal Growth Factor Receptor in prokaryotic and baculovirus expression systems.(2018) International Journal of Biological Macromolecules. 110:582-587.Read more>>
  • Hu,et al.The molecular mechanism of cell cycle arrest in the Bursa of Fabricius in chick exposed to Aflatoxin B 1.(2018) Scientific Reports. 8:1770.Read more>>
  • Linlin Liu. et al. Site specific biotinylated antibody functionalized Ag@AuNIs LSPR biosensor for the ultrasensitive detection of exosomal MCT4, a glioblastoma progression biomarker. CHEM ENG J. 2022 Oct;446:137383Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human laryngocarcinoma labeled with Anti-EGFRvIII Polyclonal Antibody, Unconjugated (bs-2558R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


A549 Cells lysates probed with EGFRvIII Polyclonal Antibody, unconjugated (bs-2558R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.


Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (EGFRvIII) polyclonal Antibody, Unconjugated (bs-2558R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.