bs-2560R [Primary Antibody]
H2AX Polyclonal Antibody
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DATASHEET

Host: Rabbit

Target Protein: H2AX

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3014

Source: KLH conjugated synthetic peptide derived from human Histone H2A

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Jul 2008].

Size: 100uL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(1:50-200)

Cross Reactive Species: Human
Mouse
Rat
Bovine
Dog
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Qi et al. Sensitization of tamoxifen-resistant breast cancer cells by Z-ligustilide through inhibiting autophagy and accumulating DNA damages. (2017) Oncotarget. 8:29300-29317Read more>>
  • Fangmei Zhang. et al. X-ray-triggered NO-released BiCSNO nanoparticles: all-in-one nano-radiosensitizer with photothermal/gas therapy for enhanced radiotherapy. 2020 Aug 31Read more>>
  • Yang, Man. et al. Mice lacking DCAF2 in placenta die at the gastrulation stage. CELL TISSUE RES. 2022 Jun;:1-14Read more>>
  • Yupeng Wang. et al. Charge-conversional click polyprodrug nanomedicine for targeted and synergistic cancer therapy. J CONTROL RELEASE. 2023 Apr;356:567Read more>>
  • Zhou Xiaohai. et al. IGF2 deficiency promotes liver aging through mitochondrial dysfunction and upregulated CEBPB signaling in d-galactose-induced aging mice. MOL MED. 2023 Dec;29(1):1-18Read more>>
  • o Qiu. et al. Association between SPRY1 and TET3 in skin photoaging and natural aging mechanisms. J COSMET DERMATOL-US. 2023 DecRead more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human glioma labeled with Anti-H2AX/Histone H2A.x Polyclonal Antibody, Unconjugated (bs-2560R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Histone H2A.x Polyclonal Antibody, Unconjugated (bs-2560R) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H2A.x) Polyclonal Antibody, Unconjugated (bs-2560R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H2A.x) Polyclonal Antibody, Unconjugated (bs-2560R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H2A.x) Polyclonal Antibody, Unconjugated (bs-2560R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H2A.x) Polyclonal Antibody, Unconjugated (bs-2560R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Lane 1: Mouse Testis tissue lysates; Lane 2: Rat Testis tissue lysates; Lane 3: Human MCF-7 cell lysates; Lane 4: Human 293T cell lysates; Lane 5: Human Jurkat cell lysates; Lane 6: Human HeLa cell lysates; Lane 7: Human K562 cell lysates; Lane 8: Human MOLT4 cell lysates probed with H2AX Polyclonal Antibody, Unconjugated (bs-2560R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.