bs-2576R [Primary Antibody]
Ly-6G Polyclonal Antibody
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Host: Rabbit

Target Protein: Ly-6G/Gr-1

Immunogen Range: 40-90/134


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 70274

Source: KLH conjugated synthetic peptide derived from mouse Ly-6G/Gr-1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Ly6G is a GPI-anchored protein, that is also known as the myeloid differentiation antigen Gr1. The antigen is transiently expressed on monocytes in the bone marrow. The level of antigen expression in the bone marrow directly correlates with granulocyte differentiation and maturation. Ly6G is expressed predominantly on neutrophils, also in a subset of eosinophils, differentiating pre-monocytes, and plasmacytoid dendritic cells.

Size: 100uL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Cross Reactive Species: Mouse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhang et al. Proteus mirabilis inhibits cancer growth and pulmonary metastasis in a mouse breast cancer model. (2017) PLoS.On. 12:e0188960Read more>>
  • Tsunashima et al. Accumulated myeloid-derived suppressor cells demonstrate distinct phenotypes and functions in two non-alcoholic steatohepatitis mouse models. (2015) Hepatobiliary.Surg.Nutr. 4:313-319Read more>>
  • Arecco et al. Elastase levels and activity are increased in dystrophic muscle and impair myoblast cell survival, proliferation and differentiation. (2016) Sci.Rep. 6:24708Read more>>
  • Ying Xu. et al. PINK1-mediated mitophagy protects against hepatic ischemia/reperfusion injury by restraining NLRP3 inflammasome activation. Free Radical Bio Med. 2020 Nov;160:871Read more>>
  • Xinhong WANG. et al. Anti-inflammatory effect of HGF responses to oral traumatic ulcers using an HGF-Tg mouse model. 2021 Nov 25Read more>>
  • Stephanie Arnold. et al. Single and Multiplex Immunohistochemistry to Detect Platelets and Neutrophils in Rat and Porcine Tissues. Methods and Protocols. 2022 Oct;5(5):71Read more>>
VALIDATION IMAGES

Mouse spleen lysates probed with Ly-6G Polyclonal Antibody, unconjugated (bs-2576R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.


Lane 1: Recombinant mouse Ly-6G protein (His-tag) probed with Ly-6G Polyclonal Antibody, Unconjugated (bs-2576R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.