bs-2577R [Primary Antibody]
NKG5 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: NKG5

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 10578

Swiss Prot: P22749

Source: KLH conjugated synthetic peptide derived from human GNLY/NKG5

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Antimicrobial protein that kills intracellular pathogens. Active against a broad range of microbes, including Gram-positive and Gram-negative bacteria, fungi, and parasites. Kills Mycobacterium tuberculosis.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 14


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

paraformaldehyde-fixed, paraffin embedded human laryngocarcinoma tissue; Antigen retrieval with 0.01M citrate buffer(pH6) boiling for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-GNLY/NKG5 Polyclonal Antibody, Unconjugated (bs-2577R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining


Formalin-fixed and paraffin embedded human larynx labeled with Rabbit Anti-GNLY/NKG5 Polyclonal Antibody, Unconjugated (bs-2577R) at 1:200 followed by conjugation to the secondary antibody and DAB staining