bs-2586R [Primary Antibody]
ASIC1/BNaC2/ASIC1A Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ASIC1/BNaC2/ASIC1A

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 41

Source: KLH conjugated synthetic peptide derived from human ASIC1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Ca(2+), Li(+) and K(+). Generates a biphasic current with a fast inactivating and a slow sustained phase. Mediates glutamate-independent Ca(2+) entry into neurons upon acidosis. This Ca(2+) overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. Functions as a postsynaptic proton receptor that influences intracellular Ca(2+) concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. Modulates activity in the circuits underlying innate fear.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Niu R et al. ASIC1a promotes synovial invasion of rheumatoid arthritis via Ca2+/Rac1 pathway. Int Immunopharmacol. 2019 Dec 19;79:106089.Read more>>
  • Yayun Xu. et al. Acid sensor ASIC1a induces synovial fibroblast proliferation via Wnt/-catenin/c-Myc pathway in rheumatoid arthritis. INT IMMUNOPHARMACOL. 2022 Dec;113:109328Read more>>
  • Xiaorui Shi. et al. Inhibiting acid-sensing ion channel exerts neuroprotective effects in experimental epilepsy via suppressing ferroptosis. CNS NEUROSCI THER. 2024 Feb;30(2):e14596Read more>>
VALIDATION IMAGES

Lane 1: Human SH-SY5Y cell lysates; Lane 2: Human 293T cell lysates; Lane 3: Human HL-60 cell lysates; Lane 4: Human U87MG cell lysates probed with ACCN2 Polyclonal Antibody, Unconjugated (bs-2586R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.