Host: Rabbit
Target Protein: Fbx32
Immunogen Range: 31-130/355
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 114907
Swiss Prot: Q969P5
Source: KLH conjugated synthetic peptide derived from human Fbx32
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 42
Cross Reactive Species:
Human
Rat
Predicted Cross Reactive Species:
Mouse
Dog
Cow
Pig
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Lin, Xuzhu, et al. "Hindlimb Immobilization, but not Castration, Induces Reduction of Undercarboxylated Osteocalcin Associated with Muscle Atrophy in Rats." Journal of Bone and Mineral Research (2016).Read more>>
- Ma X. et al. Endoplasmic Reticulum Stress Is Involved in Muscular Pathogenesis in Idiopathic Inflammatory Myopathies.. Front Cell Dev Biol. 2022 Feb;10:791986-791986Read more>>
- Zhang Quan-Bing. et al. Effect of Electrical Stimulation on Disuse Muscular Atrophy Induced by Immobilization: Correlation with Upregulation of PERK Signal and Parkin-Mediated Mitophagy. AM J PHYS MED REHAB. 2023 Jan;:10.1097/PHM.0000000000002182Read more>>
Formalin-fixed and paraffin embedded rat uterus labeled with Anti MAFbx/Fbx32 Polyclonal Antibody, Unconjugated (bs2591R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Fbx32) Polyclonal Antibody, Unconjugated (bs-2591R) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
A549 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Fbx32 Polyclonal Antibody(bs-2591R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded (human heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Fbx32) Polyclonal Antibody, Unconjugated (bs-2591R) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.