bs-2593R-A750 [Conjugated Primary Antibody]
Caspase 3 Polyclonal Antibody, ALEXA FLUOR® 750 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Caspase 3

Immunogen Range: 11-120/277


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 25402

Swiss Prot: P55213

Source: KLH conjugated synthetic peptide derived from rat Caspase 3

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage (By similarity).

Conjugation: ALEXA FLUOR® 750

Excitation/ Emission: 749nm/775nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 28


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Luo, Xiaoming, et al. "Pharmacokinetics and antitumor efficacy of micelles assembled from multiarmed amphiphilic copolymers with drug conjugates in comparison with drug-encapsulated micelles." European Journal of Pharmaceutics and Biopharmaceutics (2015).Read more>>
  • Duan, Xiaoxu, et al. "Antioxidant tert-butylhydroquinone ameliorates arsenic-induced intracellular damages and apoptosis through induction of Nrf2-dependent antioxidant responses as well as stabilization of anti-apoptotic factor Bcl− 2 in human keratinocytes." Free Radical Biology and Medicine(2016).Read more>>
  • Zhang, Xiaolin, and Hao Yu. "Matrine inhibits diethylnitrosamine-induced HCC proliferation in rats through inducing apoptosis via p53, Bax-dependent caspase-3 activation pathway and down-regulating MLCK overexpression (Supplement 2016)." Iranian Journal of Pharmaceutical Research (2016).Read more>>
  • Mingying Han. et al. Verapamil inhibits early acute liver failure through suppressing the NLRP3 inflammasome pathway. 2021 May 24Read more>>
  • Huan-Tong Wu. et al. Edaravone attenuates H2O2 or glutamate-induced toxicity in hippocampal neurons and improves AlCl3/D-galactose induced cognitive impairment in mice. Neurotoxicology. 2021 Jul;85:68Read more>>
  • Simsek, Aysel. et al. Anti-proliferative and apoptotic effects of green synthesized silver nanoparticles using Lavandula angustifolia on human glioblastoma cells. 3 Biotech. 2021 Aug;11(8):1-1Read more>>
  • Salman, Giray. et al. Anticancer Effects of Vitis vinifera L. Mediated Biosynthesized Silver Nanoparticles and Cotreatment with 5 Fluorouracil on HT-29 Cell Line. 2021 Sep 21Read more>>
  • arzegar-fallah, Anita. et al. Serotonin type-3 receptor antagonists selectively kill melanoma cells through classical apoptosis, microtubule depolymerisation, ERK activation, and NF-B downregulation. 2021 Oct 15Read more>>
  • Jun Jiang. et al. Nano-enabled photosynthesis in tumours to activate lipid peroxidation for overcoming cancer resistances. BIOMATERIALS. 2022 Jun;285:121561Read more>>
  • Uygar Sa??k. et al. Effect of ferulic acid on testicular damage caused by torsion-detorsion in rats. BIOTECH HISTOCHEM. 2022 Aug 23Read more>>
  • Can Sezer. et al. Neuroprotective Effects of Milrinone on Acute Traumatic Brain ?njury. WORLD NEUROSURG. 2022 NovRead more>>
VALIDATION IMAGES