bs-2595R [Primary Antibody]
IL-1R2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: IL-1R2

Immunogen Range: 301-398/398


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7850

Swiss Prot: P27930

Source: KLH conjugated synthetic peptide derived from human IL-1R II

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Non-signaling receptor for IL1A, IL1B and IL1RN. Reduces IL1B activities. Serves as a decoy receptor by competetive binding to IL1B and preventing its binding to IL1R1. Also modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. IL1R2 (membrane and secreted forms) preferentially binds IL1B and poorly IL1A and IL1RN. The secreted IL1R2 recruits secreted IL1RAP with high affinity; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:500-2000)
ELISA(1:5000-10000)
FCM(1:100)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(IHC-P)(1:100-500)
IF(IHC-F)(1:100-500)
IF(ICC)(1:100-500)

Predicted Molecular Weight: 44


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Mouse lung lysates probed with Anti-IL-1R2 Polyclonal Antibody (bs-2595R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.\n


RSC96 cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IL-1R2 Antibody(bs-2595R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).


Lane 1: SW480 cell lysates probed with IL-1R2 Polyclonal Antibody, Unconjugated (bs-2595R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.