KLH conjugated synthetic peptide derived from human IL-1R II
Purified by Protein A.
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Non-signaling receptor for IL1A, IL1B and IL1RN. Reduces IL1B activities. Serves as a decoy receptor by competetive binding to IL1B and preventing its binding to IL1R1. Also modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. IL1R2 (membrane and secreted forms) preferentially binds IL1B and poorly IL1A and IL1RN. The secreted IL1R2 recruits secreted IL1RAP with high affinity; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors.
Mouse lung lysates probed with Anti-IL-1R2 Polyclonal Antibody (bs-2595R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.\n
RSC96 cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with
IL-1R2 Antibody（bs-2595R） at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).
Lane 1: SW480 cell lysates probed with IL-1R2 Polyclonal Antibody, Unconjugated (bs-2595R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.