Host: Rabbit
Target Protein: IL-22
Immunogen Range: 35-179/179
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 50616
Swiss Prot: Q9GZX6
Source: KLH conjugated synthetic peptide derived from human IL-22
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 17
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species: Dog
For research use only. Not intended for diagnostic or therapeutic use.
- Huang, Yong-Hong, et al. "Th22 cell accumulation is associated with colorectal cancer development."World Journal of Gastroenterology Read more>>
- Karaboga, İhsan, Selim Demirtas, and Turan Karaca. "Investigation of the relationship between the Th17/IL-23 pathway and innate-adaptive immune system in TNBS-induced colitis in rats." Iranian Journal of Basic Medical Sciences 20.8 (2017): 870-879.Read more>>
- Zhang, Yan, and Mikhail G. Kolonin. "Cytokine signaling regulating adipose stromal cell trafficking." Adipocyte 5.4 (2016): 369-374.Read more>>
- Karaboga et al. Investigation of the relationship between the Th17/IL-23 pathway and innate-adaptive immune system in TNBS-induced colitis in rats. (2017) Iran.J.Basic.Med.Sci. 20:870-879Read more>>
- Wanzhen Li. et al. Ac2-26 attenuates hepatic ischemia-reperfusion injury in mice via regulating IL-22/IL-22R1/STAT3 signaling. PEERJ. 2022 Sep;10:e14086Read more>>
- Jieying Chen. et al. Nestin+ Peyer's patch resident MSCs enhance healing of inflammatory bowel disease through IL-22-mediated intestinal epithelial repair. CELL PROLIFERAT. 2022 Nov;:e13363Read more>>
- Jing Li. et al. Salidroside Inhibits Ganglion Cell Apoptosis by Suppressing the Mller Cell Inflammatory Response in Diabetic Retinopathy. CURR EYE RES. 2023 Jun 07Read more>>
- Zhengchen Jiang. et al. IL-22 relieves hepatic ischemia-reperfusion injury by inhibiting mitochondrial apoptosis based on the activation of STAT3. INT J BIOCHEM CELL B. 2024 Jan;166:106503Read more>>
- Yu-Qing Hu. et al. Unbalanced Aryl Hydrocarbon Receptor Expression in Peripheral and Lesional T Cell Subsets of Atopic Dermatitis. CLIN COSMET INV DERM. 2023 Dec 31Read more>>
- Feng-Ling Tang. et al. Fraxin (7-hydroxy-6-methoxycoumarin 8-glucoside) confers protection against ionizing radiation-induced intestinal epithelial injury in vitro and in vivo. INT IMMUNOPHARMACOL. 2024 Mar;129:111637Read more>>
Paraformaldehyde-fixed, paraffin embedded mouse tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with IL-22 Polyclonal Antibody (bs-2623R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Lane 1: A549 cell lysates probed with IL-22 Polyclonal Antibody, Unconjugated (bs-2623R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human 293T cell lysates; Lane 2: Human A549 cell lysates probed with IL-22 Polyclonal Antibody, Unconjugated (bs-2623R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.