bs-2636R [Primary Antibody]
Integrin alpha 2b Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Integrin alpha 2b/CD41

Immunogen Range: 351-450/1033


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 16399

Swiss Prot: Q9QUM0

Source: KLH conjugated synthetic peptide derived from mouse Integrin alpha 2b

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 125


Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse
Cow
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Mei, Yun, et al. "The regulatory role of the adrenergic agonists phenylephrine and isoproterenol on fetal hemoglobin expression and erythroid differentiation." Endocrinology (2013): en-2013.Read more>>
  • Araki, Tomoya, et al. "Augmented EPR effect by photo-triggered tumor vascular treatment improved therapeutic efficacy of liposomal paclitaxel in mice bearing tumors with low permeable vasculature." Journal of Controlled Release (2015).Read more>>
  • Lê, Vuong Ba, et al. "Platelet Activation and Aggregation Promote Lung Inflammation and Influenza Virus Pathogenesis." American Journal of Respiratory and Critical Care Medicine (2015).Read more>>
  • Sugiyama et al. Influenza Virus Infection Induces Platelet-Endothelial Adhesion Which Contributes to Lung Injury. (2016) J.Viro. 90:1812-23Read more>>
  • Saleh T et al. Conjugating homogenized liver\extracellular matrix into decellularized hepatic scaffold for liver tissue engineering. J Biomed Mater Res A. 2020 Oct;108(10):1991-2004.Read more>>
VALIDATION IMAGES

HL-60 cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Integrin alpha 2b Antibody(bs-2636R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).