bs-2637R [Primary Antibody]
ERK1 + 2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ERK1 + 2

Immunogen Range: 251-358/358


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 26413

Swiss Prot: P63085

Source: KLH conjugated synthetic peptide derived from mouse ERK2

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage: Store at -20°C for 12 months..

Background:

The protein encoded by this gene is a member of the MAPkinase family. MAP kinases, also known as extracellularsignal-regulated kinases (ERKs), act in a signaling cascade thatregulates various cellular processes such as proliferation,differentiation, and cell cycle progression in response to avariety of extracellular signals. This kinase is activated byupstream kinases, resulting in its translocation to the nucleuswhere it phosphorylates nuclear targets. Alternatively splicedtranscript variants encoding different protein isoforms have beendescribed. [provided by RefSeq, Jul 2008].

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-1000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 42/44


Cross Reactive Species: Human
Mouse
Rat
Chicken
Rabbit
Others

Predicted Cross Reactive Species: Dog
Cow
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Dang, Hui-Min, et al. "“Qufeng Tongluo” acupuncture Prevents the progression of glomerulonephritis by Decreasing renal sympathetic nerve activity." Journal of Ethnopharmacology (2014).Read more>>
  • Wang T, Zhao L, Guo Y, Zhang M, Pei H (2015) Picroside II Inhibits Neuronal Apoptosis and Improves the Morphology and Structure of Brain Tissue following Cerebral Ischemic Injury in Rats. PLoS ONE 10(4): e0124099.Read more>>
  • Wang, Tingting, et al. "Picroside II has a neuroprotective effect by inhibiting ERK1/2 activation after cerebral ischemic injury in rats." Clinical and Experimental Pharmacology and Physiology (2015).Read more>>
  • Chen, Wei, et al. "Mycobacterium tuberculosis PE25/PPE41 protein complex induces activation and maturation of dendritic cells and drives Th2-biased immune responses." Medical Microbiology and Immunology (2015): 1-13.Read more>>
  • Zhao, Hongyu, et al. "Betulin attenuates lung and liver injuries in sepsis."International Immunopharmacology 30 (2016): 50-56.Read more>>
  • Cong, Lin, and Wenting Chen. "Neuroprotective Effect of Ginsenoside Rd on Spinal Cord Injury Rats." Basic & Clinical Pharmacology & Toxicology(2016).Read more>>
  • Zhao, Haiyan, et al. "Inhibition of endocan attenuates monocrotaline-induced connective tissue disease related pulmonary arterial hypertension." International Immunopharmacology 42 (2017): 115-121.Read more>>
  • Yu, Haijie, et al. "Gypenoside Protects Cardiomyocytes against Ischemia-Reperfusion Injury via the Inhibition of Mitogen-Activated Protein Kinase Mediated Nuclear Factor Kappa B Pathway In Vitro and In Vivo." Frontiers in Pharmacology 7 (2016).Read more>>
  • Li, Jing, et al. "Downregulation of BRAF‑activated non‑coding RNA suppresses the proliferation, migration and invasion, and induces apoptosis of hepatocellular carcinoma cells." Oncology Letters 14.4 (2017): 4751-4757.Read more>>
  • Liu, Xinwei, et al. "Inhibition of BTK protects lungs from trauma-hemorrhagic shock-induced injury in rats." Molecular Medicine Reports 16.1 (2017): 192-200.Read more>>
  • Zhang, Fengwei, et al. "Seasonal Expression of Oxytocin and Oxytocin Receptor in the Scented Gland of Male Muskrat (Ondatra zibethicus)." Scientific Reports 7.1 (2017): 16627.Read more>>
  • Xu et al. RKTG overexpression inhibits proliferation and induces apoptosis of human leukemia cells via suppression of the ERK and PI3K/AKT signaling pathways. (2017) Oncol.Let. 14:965-970Read more>>
  • Zhao et al. Adipose differentiation‑related protein knockdown inhibits vascular smooth muscle cell proliferation and migration and attenuates neointima formation. (2017) Mol.Med.Re. 16:3079-3086Read more>>
  • Li et al. Activation of the Extracellular Signal-Regulated Kinase Signaling Is Critical for Human Umbilical Cord Mesenchymal Stem Cell Osteogenic Differentiation. (2016) Biomed.Res.In. 2016:3764372Read more>>
  • Chen et al. Downregulation of MACC1 expression enhances cisplatin sensitivity in SKOV-3/DDP cells. (2015) Genet.Mol.Re. 14:17134-44Read more>>
  • Zheng et al. Ginsenoside Rb1 improves cardiac function and remodeling in heart failure. (2017) Exp.Ani. 66:217-228Read more>>
  • Li et al. Downregulation of BRAF-activated non-coding RNA suppresses the proliferation, migration and invasion, and induces apoptosis of hepatocellular carcinoma cells. (2017) Oncol.Lett. 14:4751-4757Read more>>
  • Cong and Chen Neuroprotective Effect of Ginsenoside Rd on Spinal Cord Injury Rats. (2016) Basic.Clin.Pharmacol.Toxicol. Read more>>
  • Lin X et al. TFF3 Contributes to Epithelial-Mesenchymal Transition (EMT) in Papillary Thyroid Carcinoma Cells via the MAPK/ERK Signaling Pathway.(2018)J Cancer;Oct 31;9(23):4430-4439. Read more>>
  • Yue H et al. Gestational exposure to PM2.5 impairs vascularization of the placenta. Sci Total Environ. 2019 May 15;665:153-161. Read more>>
  • Liu W et al. Synergistic effect of tolfenamic acid and glycyrrhizic acid on TPA-induced skin inflammation in mice. MedChemComm.2019.Read more>>
  • Imoto K et al. Periostin Mediates Right Ventricular Failure through Induction of Inducible Nitric Oxide SynthaseExpression in Right Ventricular Fibroblasts from Monocrotaline-Induced Pulmonary Arterial Hypertensive Rats. Int J Mol Sci. 2018 Dec 24;20(1). pii: E62.Read more>>
  • Li QH et al. Effect of heat stress on mitogen-activated protein kinases in the hypothalamic− pituitary− gonadal axis of developing Wenchang chicks. Poultry Science.2019.Read more>>
  • Zhou J et al. Paeonol antagonizes oncogenesis of osteosarcoma by inhibiting the function of TLR4/MAPK/NF-κB pathway. Acta Histochem. 2019 Oct 3:151455. Read more>>
  • Ma Q et al. Vitamin B5 inhibit RANKL induced osteoclastogenesis and ovariectomy induced osteoporosis by scavenging ROS generation. Am J Transl Res. 2019 Aug 15;11(8):5008-5018. eCollection 2019.Read more>>
  • Liu F et al. Clinical and biological significances of heat shock protein 90 (Hsp90) in human nasopharyngeal carcinoma cells and anti-cancer effects of Hsp90 inhibitor. Biomed Pharmacother. 2019 Oct 18;120:109533. Read more>>
  • Zhang J et al. Down‐regulation of Suv39h1 attenuates neointima formation after carotid artery injury in diabetic rats. J Cell Mol Med. 2019 Nov 17. Read more>>
  • Shin WS et al. Catalytically inactive receptor tyrosine kinase PTK7 activates FGFR1 independent of FGF. FASEB J. 2019 Sep 18:fj201900932R. Read more>>
  • Zhu J et al. SPARC Promotes Self‐Renewal of Limbal Epithelial Stem Cells and Ocular Surface Restoration through JNK and p38‐MAPK Signaling Pathways. Stem Cells. 2019 Oct 23.Read more>>
  • Gu F et al. Lactobacillus casei improves depression-like behavior in chronic unpredictable mild stress-induced rats by the BDNF-TrkB signal pathway and the intestinal microbiota. Food Funct. 2020 Jul 1;11(7):6148-6157.Read more>>
  • Jian Wang. et al. Lactobacillus salivarius ameliorated Mycoplasma gallisepticum-induced inflammatory injury and secondary Escherichia coli infection in chickens: Involvement of intestinal microbiota. Vet Immunol Immunop. 2021 Mar;233:110192Read more>>
  • Wenbo Ge. et al. 17-estradiol protects sheep oviduct epithelial cells against lipopolysaccharide-induced inflammation in vitro. Mol Immunol. 2020 Nov;127:21Read more>>
  • Jian Wang. et al. A respiratory commensal bacterium acts as a risk factor for Mycoplasma gallisepticum infection in chickens. Vet Immunol Immunop. 2020 Dec;230:110127Read more>>
  • Meixuan Chen. et al. Activation of the opioid receptor relieves cerebral ischemic injury in rats via EGFR transactivation. Life Sci. 2021 May;273:119292Read more>>
  • Dongyi Xu. et al. The Critical Role of NLRP6 Inflammasome in Streptococcus pneumoniae Infection In Vitro and In Vivo. Int J Mol Sci. 2021 Jan;22(8):3876Read more>>
  • Qihe Tang. et al. Bergenin Monohydrate Attenuates Inflammatory Response via MAPK and NF-B Pathways Against Klebsiella pneumonia Infection. Front Pharmacol. 2021; 12: 651664Read more>>
  • Jianye Yang. et al. Astragalus polysaccharide attenuates LPS-related inflammatory osteolysis by suppressing osteoclastogenesis by reducing the MAPK signalling pathway. 2021 Jun 02Read more>>
  • Ai Jin. et al. TSLP-induced collagen type-I synthesis through STAT3 and PRMT1 is sensitive to calcitriol in human lung fibroblasts. Bba-Mol Cell Res. 2021 Jun;:119083Read more>>
  • Lili Liu. et al. Rutin Ameliorates Cadmium-Induced Necroptosis in the Chicken Liver via Inhibiting Oxidative Stress and MAPK/NF-B Pathway. 2021 Jun 06Read more>>
VALIDATION IMAGES

A549 cell lysates probed with Rabbit Anti-ERK1 + 2 Polyclonal Antibody, Unconjugated (bs-2673R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.


Lane 1: mouse heart lysates Lane 2: mouse brain lysates probed with Anti ERK1/2(p42/p42 MAPK) Polyclonal Antibody, Unconjugated (bs-2637R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 42kD. Observed band size: 42kD.


Lane 1: Mouse Kidney lysates; Lane 2: Mouse Liver lysates probed with ERK1 + 2 Polyclonal Antibody, Unconjugated (bs-2637R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: A431 cell Lysate;Lane 2: Mouse Cerebrum Lysate;Lane 3: Rat Cerebrum Lysate;Lane 4: Mouse Lymph node Lysate;Lane 5: Rat Lymph node Lysate;Lane 6: SiHa cell Lysate;Lane 7: HUVEC cell Lysate probed with ERK1 + ERK2 Polyclonal Antibody, Unconjugated (bs-2637R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C


Tissue/cell: HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (ERK1 + ERK2) Polyclonal Antibody, Unconjugated (bs-2637R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.


Tissue/cell: HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (ERK1 + ERK2) Polyclonal Antibody, Unconjugated (bs-2637R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.


Lane 1: Mouse Spleen lysates; Lane 2: Mouse Lymph node lysates probed with ERK1 + 2 Polyclonal Antibody, Unconjugated (bs-2637R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.