Host: Rabbit
Target Protein: SPHK1
Immunogen Range: 1-100/384
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 8877
Swiss Prot: Q9NYA1
Source: KLH conjugated synthetic peptide derived from human SPHK1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 42-52
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Tran, Hai B., et al. "Cigarette smoke inhibits efferocytosis via deregulation of sphingosine kinase signaling: reversal with exogenous S1P and the S1P analogue FTY720." Journal of Leukocyte Biology (2016): jlb-3A1015.Read more>>
- Tani, Kohsuke, Keiji Tabuchi, and Akira Hara. "Hair Cell Loss Induced by Sphingosine and a Sphingosine Kinase Inhibitor in the Rat Cochlea."Neurotoxicity research 29.1 (2016): 35-46.Read more>>
- Tran, Hai B., et al. "Disrupted epithelial/macrophage crosstalk via Spinster homologue 2-mediated S1P signaling may drive defective macrophage phagocytic function in COPD." PloS one 12.11 (2017): e0179577.Read more>>
- Harl Guillaume. et al. Macroautophagy in lymphatic endothelial cells inhibits T cellCmediated autoimmunity. J Exp Med. 2021 Jun;218(6):e20201776.Read more>>
- Hai B. Tran. et al. Dysregulated zinc and sphingosine-1-phosphate signaling in pulmonary hypertension: Potential effects by targeting of bone morphogenetic protein receptor type 2 in pulmonary microvessels. 2021 Aug 15Read more>>
- Christopher D. Carlucci. et al. Resveratrol Protects against Skin Inflammation through Inhibition of Mast Cell, Sphingosine Kinase-1, Stat3 and NF-κB p65 Signaling Activation in Mice. INT J MOL SCI. 2023 Jan;24(7):6707Read more>>
Mouse heart lysates probed with SPHK1 Polyclonal Antibody, unconjugated (bs-2652R) at 1:30 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.\n
Lane 1: Mouse Pancreas tissue lysates; Lane 2: Mouse Lung tissue lysates; Lane 3: Rat Kidney tissue lysates probed with SPHK1 Polyclonal Antibody, Unconjugated (bs-2652R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.