bs-2662R [Primary Antibody]
MD-2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: MD-2

Immunogen Range: 81-160/160


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 23643

Source: KLH conjugated synthetic peptide derived from human LY-96

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Cooperates with TLR4 in the innate immune response to bacterial lipopolysaccharide (LPS), and with TLR2 in the response to cell wall components from Gram-positive and Gram-negative bacteria. Enhances TLR4-dependent activation of NF-kappa-B. Cells expressing both MD2 and TLR4, but not TLR4 alone, respond to LPS.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 16


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat
Cow
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhou S et al. TLR 4 increases the stemness and is highly expressed in relapsed human hepatocellular carcinoma. Cancer Med. 2019 Apr 8. Read more>>
  • Guirong Chen. et al. The interaction of MD-2 with small molecules in huanglian jiedu decoction play a critical role in the treatment of sepsis.. FRONT PHARMACOL. 2022 Sep;13:947095-947095Read more>>
VALIDATION IMAGES

Lane 1: Human U87MG cell lysates; Lane 2: Human HUVEC cell lysates; Lane 3: Human U251 cell lysates probed with MD2 Polyclonal Antibody, Unconjugated (bs-2662R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.