bs-2670R [Primary Antibody]
Tubb3 Polyclonal Antibody
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Host: Rabbit

Target Protein: Tubb3

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 10381

Source: KLH conjugated synthetic peptide derived from human Tubulin beta 3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Neuronal MarkerBeta III tubulin is abundant in the central and peripheral nervous systems (CNS and PNS) where it is prominently expressed during fetal and postnatal development. As exemplified in cerebellar and sympathoadrenal neurogenesis, the distribution of beta III is neuron-associated, exhibiting distinct temporospatial gradients according to the regional neuroepithelia of origin. However, transient expression of this protein is also present in the subventricular zones of the CNS comprising putative neuronal- and/or glial precursor cells, as well as in Kulchitsky neuroendocrine cells of the fetal respiratory epithelium. This temporally restricted, potentially non-neuronal expression may have implications in the identification of presumptive neurons derived from embryonic stem cells.

Size: 100µL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(1:50-200)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Li, Wen-Jing, et al. "Systematic expression analysis of genes related to multidrug-resistance in isogenic docetaxel-and adriamycin-resistant breast cancer cell lines." Molecular biology reports (2013): 1-8.Read more>>
  • Qu, Xinjian, et al. "Differentiation of reprogrammed human adipose mesenchymal stem cells toward neural cells with defined transcription factors." Biochemical and Biophysical Research Communications (2013).Read more>>
VALIDATION IMAGES

Lane 1: Mouse Brain lysates; Lane 2: Mouse Cerebellum lysates; Lane 3: Rat Brain lysates; Lane 4: Rat Cerebellum lysates; 5: Human SH-SY5Y cell lysates. Probed with Tubb3 polyclonal Antibody, unconjugated (bs-2670R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.