bs-2673R
[Primary Antibody]
C5b-9 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: C5b-9
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 735
Swiss Prot: P02748
Source: Purified Human SC5b-9 Complex
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C9 is the pore-forming subunit of the MAC.
Size: 100ul
Concentration: 1ug/ul
Applications:
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Ücal, Muammer, et al. "Widespread cortical demyelination of both hemispheres can be induced by injection of pro-inflammatory cytokines via an implanted catheter in the cortex of MOG-immunized rats." Experimental Neurology (2017).Read more>>
- Arias et al. Disruption of CD8+ Treg activity results in expansion of T follicular helper cells and enhanced antitumor immunity. (2014) Cancer.Immunol.Res. 2:207-16Read more>>
- Zhao et al. Age-related increases in amyloid beta and membrane attack complex: evidence of inflammasome activation in the rodent eye. (2015) J.Neuroinflammation. 12:121Read more>>
- Huang et al. C5b-9-Targeted Molecular MR Imaging in Rats with Heymann Nephritis: A New Approach in the Evaluation of Nephrotic Syndrome. (2015) PLoS.One. 10:e0121244Read more>>
- Yang W et al. The role of protein disulphide-isomerase A3 as autoantigen in the pathogenesis of autoimmune thyroiditis and related brain damage in adult mice. Clin Immunol. 2020 Jan 23;212:108350. Read more>>
- Yang W et al. The role of protein disulphide-isomerase A3 as autoantigen in the pathogenesis of autoimmune thyroiditis and related brain damage in adult mice. Clin Immunol. 2020 Jan 23;212:108350. Read more>>
- Akiko Takahata. et al. Crucial Role of AIM/CD5L in the Development of Glomerular Inflammation in IgA Nephropathy. J Am Soc Nephrol. 2020 Sep;31(9):2013-2024Read more>>
- Liu, Tao. et al. Microarray-based analysis of renal complement components reveals a therapeutic target for lupus nephritis. Arthritis Res Ther. 2021 Dec;23(1):1-15Read more>>
- Keng Chen. et al. Complement factor B inhibitor LNP023 improves lupus nephritis in MRL/lpr mice. BIOMED PHARMACOTHER. 2022 Sep;153:113433Read more>>
- Wenbin Yang. et al. IL-1-dependent extravasation of preexisting lung-restricted autoantibodies during lung transplantation activates complement and mediates primary graft dysfunction. J CLIN INVEST. 2022 Oct 17;132(20):e157975Read more>>
- Chen, Yujie. et al. GSDME deficiency leads to the aggravation of UVB-induced skin inflammation through enhancing recruitment and activation of neutrophils. CELL DEATH DIS. 2022 Oct;13(10):1-14Read more>>
- Hao-jie Zhang. et al. MAC mediates mammary duct epithelial cell injury in plasma cell mastitis and granulomatous mastitis. INT IMMUNOPHARMACOL. 2022 Dec;113:109303Read more>>
- Tsuge Mitsuru. et al. Neonatal hemochromatosis with -thalassemia: a case report and analysis of serum iron regulators. BMC PEDIATR. 2022 Dec;22(1):1-7Read more>>
- Kenichi Serizawa. et al. Intradermal AQP4 peptide immunization induces clinical features of neuromyelitis optica spectrum disorder in mice. J NEUROIMMUNOL. 2023 Jul;380:578109Read more>>
- Hu Mengyan. et al. Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model. NAT COMMUN. 2023 Jul;14(1):1-19Read more>>
- Suzanne M. Green-Fulgham. et al. Voluntary wheel running prevents formation of membrane attack complexes and myelin degradation after peripheral nerve injury. BRAIN BEHAV IMMUN. 2023 NovRead more>>
- Hiyamizu Shion. et al. Forensic significance of intracardiac expressions of Nrf2 in acute myocardial ischemia. SCI REP-UK. 2024 Feb;14(1):1-9Read more>>
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with C5b-9 Polyclonal Antibody, Unconjugated (bs-2673R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human rectal cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with C5b-9 Polyclonal Antibody, Unconjugated (bs-2673R) at 1:100 overnight at 4°C, followed by secondary and DAB staining.