bs-2677R [Primary Antibody]
LRP1/CD91 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: LRP1/CD91

Immunogen Range: 4451-4545/4545


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4035

Swiss Prot: Q07954

Source: KLH conjugated synthetic peptide derived from human CD91

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Endocytic receptor involved in endocytosis and in phagocytosis of apoptotic cells. Required for early embryonic development. Involved in cellular lipid homeostasis. Involved in the plasma clearance of chylomicron remnants and activated LRPAP1 (alpha 2-macroglobulin), as well as the local metabolism of complexes between plasminogen activators and their endogenous inhibitors. May modulate cellular events, such as APP metabolism, kinase-dependent intracellular signaling, neuronal calcium signaling as well as neurotransmission. Functions as a receptor for Pseudomonas aeruginosa exotoxin A.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 85/515


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Pig
Horse
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Turco et al. Specific renal parenchymal-derived urinary extracellular vesicles identify age-associated structural changes in living donor kidneys. (2016) J.Extracell.Vesicle. 5:29642Read more>>
  • Tetsuya Shimokawa. et al. Prosaposin in the rat oviductal epithelial cells. 2020 Nov 26Read more>>
VALIDATION IMAGES

Human U937 cells probed with \tLRP1/CD91 Polyclonal Antibody, Unconjugated (bs-2677R) at 1:100 for 30 minutes followed by incubation with a PE conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).


Formalin-fixed and paraffin embedded mouse lung cancer labeled with Anti-LRP1/CD91 Polyclonal Antibody, Unconjugated (bs-2677R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded mouse brain labeled with Anti-LRP1/CD91 Polyclonal Antibody, Unconjugated (bs-2677R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LRP1/CD91) Polyclonal Antibody, Unconjugated (bs-2677R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.