bs-2688R [Primary Antibody]
ApoA4 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ApoA4

Immunogen Range: 21-120/396


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 337

Swiss Prot: P06727

Source: KLH conjugated synthetic peptide derived from human ApoA4

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Predicted Molecular Weight: 41


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Rabbit Anti ApoA4 Polyclonal Antibody, Unconjugated (bs-2688R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded rat brain carcinoma labeled with Rabbit Anti-ApoA4 Polyclonal Antibody, Unconjugated (bs-2688R) at 1:200 followed by secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (bs-0295G-Cy3) used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml) was used to stain the cell nuclei


Lane 1: mouse heart lysates Lane 2: mouse kidney lysates probed with Anti APOA4 Polyclonal Antibody, Unconjugated (bs-2688R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 41kD. Observed band size: 41kD


Paraformaldehyde-fixed, paraffin embedded Mouse intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ApoA4 Polyclonal Antibody, Unconjugated (bs-2688R) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Rat intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ApoA4 Polyclonal Antibody, Unconjugated (bs-2688R) at 1:200 overnight at 4°C, DAB staining.


Lane 1: Rat Kidney lysates; Lane 2: Rat Liver lysates probed with ApoA4 Polyclonal Antibody, Unconjugated (bs-2688R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.