Host: Rabbit
Target Protein: MCT4
Immunogen Range: 401-465/465
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 9123
Swiss Prot: O15427
Source: KLH conjugated synthetic peptide derived from human MCT4
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 52
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Horse
Chicken
For research use only. Not intended for diagnostic or therapeutic use.
- Prado-Garcia H et al. Lactic Acidosis in the Presence of Glucose Diminishes Warburg Effect in Lung Adenocarcinoma Cells. Front Oncol. 2020 Jun 12;10:807.Read more>>
- Carolina Manosalva. et al. D-Lactate Increases Cytokine Production in Bovine Fibroblast-Like Synoviocytes via MCT1 Uptake and the MAPK, PI3K/Akt, and NFB Pathways. Animals-Basel. 2020 Nov;10(11):2105Read more>>
- Qingxian Li. et al. Glucocorticoid caused lactic acid accumulation and damage in human chondrocytes via ROS-mediated inhibition of Monboxylate Transporter 4. Bone. 2021 Dec;:116299Read more>>
- Xiao Han. et al. ZEB1 induces ROS generation through directly promoting MCT4 transcription to facilitate breast cancer. Exp Cell Res. 2022 Mar;412:113044Read more>>
- Ruofan Yao. et al. Maternal obesity-associated disruption of polarized lactate transporter MCT4 expression in human placenta. REPROD TOXICOL. 2022 Sep;112:1Read more>>
- P.P.M. Scariot. et al. Monocarboxylate transporters (MCTs) in skeletal muscle and hypothalamus of less or more physically active mice exposed to aerobic training. LIFE SCI. 2022 Oct;307:120872Read more>>
- Pedro Paulo Menezes Scariot. et al. Living high - training low model applied to C57BL/6J mice: Effects on physiological parameters related to aerobic fitness and acid-base balance. LIFE SCI. 2023 Mar;317:121443Read more>>
Formalin-fixed and paraffin embedded rat brain labeled with Anti-MCT4 Polyclonal Antibody, Unconjugated (bs-2698R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat stomach labeled with Anti-MCT4 Polyclonal Antibody, Unconjugated (bs-2698R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-SLC16A3 Polyclonal Antibody, Unconjugated(bs-2698R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 rat kidney lysates, L2 rat brain lysates probed with Anti- SLC16A3/MCT4 Polyclonal Antibody, Unconjugated (bs-2698R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band and observed band size: 51kD
Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MCT4 Polyclonal Antibody, Unconjugated (bs-2698R) at 1:400 overnight at 4°C, DAB staining.
Lane 1: Mouse Muscle lysates probed with MCT4 Polyclonal Antibody, Unconjugated (bs-2698R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.