bs-2711R [Primary Antibody]
IL-27RA Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: IL-27RA

Immunogen Range: 21-120/636


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 9466

Swiss Prot: Q6UWB1

Source: KLH conjugated synthetic peptide derived from human IL-27RA

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Receptor for IL27. Requires IL6ST/gp130 to mediate signal transduction in response to IL27. This signaling system acts through STAT3 and STAT1. Involved in the regulation of Th1-type immune responses. Also appears to be involved in innate defense mechanisms.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 70


Cross Reactive Species: Human
Mouse
Others

Predicted Cross Reactive Species: Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Xu, Fenghuang, et al. "IL-27 regulates the adherence, proliferation, and migration of MSCs and enhances their regulatory effects on Th1 and Th2 subset generations." Immunologic Research (2017): 1-10.Read more>>
  • Shanshan Zhao. et al. IL\27 R+ cells promoted allorejection via enhancing STAT1/3/5 phosphorylation. J Cell Mol Med. 2020 Sep;24(18):10756-10767Read more>>
  • Shanshan Zhao. et al. IL\27 R+ cells promoted allorejection via enhancing STAT1/3/5 phosphorylation. J Cell Mol Med. 2020 Sep;24(18):10756-10767Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded mouse lymphoma labeled with Rabbit Anti IL-27R/TCCR Polyclonal Antibody, Unconjugated (bs-2711R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Antigen: bs-2711P, 0.2ug/100ul \nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\nTMB staining;\nRead the data in MicroplateReader by 450