Host: Rabbit
Target Protein: TREM2
Immunogen Range: 1-100/230
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 54209
Swiss Prot: Q9NZC2
Source: KLH conjugated synthetic peptide derived from human TREM2
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 25
Cross Reactive Species:
Human
Mouse
Rat
Pig
Predicted Cross Reactive Species:
Dog
Cow
Horse
For research use only. Not intended for diagnostic or therapeutic use.
- Satoh, Jun-ichi, et al. "A survey of TREM2 antibodies reveals neuronal but not microglial staining in formalin-fixed paraffin-embedded postmortem Alzheimer's brain tissues." Alzheimer's research & therapy 5.4 (2013): 30.Read more>>
- Rai et al. Vitamin D attenuates inflammation, fatty infiltration, and cartilage loss in the knee of hyperlipidemic microswine. (2016) Arthritis.Res.The. 18:203Read more>>
- Li et al. TREM2 inhibits inflammatory responses in mouse microglia by suppressing the PI3K/NF-κB signaling. (2018) Cell.Biol.Int. Apr 16.Read more>>
- Elizabeth Olmsted-Davis. et al. A Population of M2 Macrophages Associated With Bone Formation. Front Immunol. 2021; 12: 686769Read more>>
- Dai Shi. et al. Synthesis and Evaluation of 68Ga-NOTA-COG1410 Targeting to TREM2 of TAMs as a Specific PET Probe for Digestive Tumor Diagnosis. Anal Chem. 2022;94(9):3819383Read more>>
- Ke Wu. et al. The microglial innate immune receptors TREM-1 and TREM-2 in the anterior cingulate cortex (ACC) drive visceral hypersensitivity and depressive-like behaviors following DSS-induced colitis. BRAIN BEHAV IMMUN. 2023 JuRead more>>
- Cheng Wenchao. et al. The Impact of Dipyridamole on Disease-Associated Microglia Phenotypic Transformation in White Matter Lesions Induced by Chronic Cerebral Hypoperfusion. NEUROCHEM RES. 2023 Dec;:1-14Read more>>
Raje cell lysates probed with Anti-TREM2 Polyclonal Antibody, Unconjugated (bs-2723R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.
Formalin-fixed and paraffin embedded human gastritis labeled with Rabbit Anti TREM2 Polyclonal Antibody, Unconjugated (bs-2723R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TREM2 Polyclonal Antibody, Unconjugated (bs-2723R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TREM2 Polyclonal Antibody, Unconjugated (bs-2723R) at 1:400 overnight at 4°C, DAB staining.
Lane 1: MCF-7 cell lysates; Lane 2: Raw264.7 cell lysates probed with TREM2 Polyclonal Antibody, Unconjugated (bs-2723R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Cerebrum lysates probed with TREM2 Polyclonal Antibody, Unconjugated (bs-2737R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TREM2) Polyclonal Antibody, Unconjugated (bs-2723R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Raw264.7 cell lysates; Lane 3: Mouse BV2 cell lysates; Lane 4: Human HepG2 cell lysates probed with TREM2 Polyclonal Antibody, Unconjugated (bs-2723R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.