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Host: Rabbit
Target Protein: Bcl-6
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 604
Swiss Prot: P41182
Source: KLH conjugated synthetic peptide derived from human Bcl-6
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100µL
Concentration: 1mg/ml
Predicted Molecular Weight: 78
Cross Reactive Species:
Human
Mouse
For research use only. Not intended for diagnostic or therapeutic use.
- Szabó, Krisztina, et al. "Expansion of circulating follicular T helper cells associates with disease severity in childhood atopic dermatitis." Immunology Letters (2017).Read more>>
- Gong, Qiaoqiao, et al. "Increased levels of CCR7 (lo) PD‑1 (hi) CXCR5+ CD4+ T cells, and associated factors Bcl‑6, CXCR5, IL‑21 and IL‑6 contribute to repeated implantation failure." Experimental and Therapeutic Medicine 14.6 (2017): 5931-5941.Read more>>
- Gong et al. Increased levels of CCR7(lo)PD-1(hi) CXCR5+CD4+T cells, and associated factors Bcl-6, CXCR5, IL-21 and IL-6 contribute to repeated implantation failure. (2017) Exp.Ther.Med. 14:5931-5941Read more>>
- Shuang Zhang. et al. Knockdown of miR?205?5p alleviates the inflammatory response in allergic rhinitis by targeting B?cell lymphoma 6. Mol Med Rep. 2021 Nov;24(5):1-11Read more>>
- Genxi Zhang. et al. miRNA-10a-5p Targeting the BCL6 Gene Regulates Proliferation, Differentiation and Apoptosis of Chicken Myoblasts. INT J MOL SCI. 2022 Jan;23(17):9545Read more>>
- Yifei Deng. et al. Astragalus polysaccharides ameliorates experimental colitis by regulating memory B cells metabolism. CHEM-BIOL INTERACT. 2024 May;394:110969Read more>>
Lane 1: Human RAMOS cell lysates; Lane 2: Human Raji cell lysates; Lane 3: Human Jurkat cell lysates probed with Bcl-6 Polyclonal Antibody, Unconjugated (bs-2734R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Bcl6) Polyclonal Antibody, Unconjugated (bs-2734R) at 1:25 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
25 ug total protein per lane of various lysates (see on figure) probed with Bcl-6 polyclonal antibody, unconjugated (bs-2734R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Bcl-6 Polyclonal Antibody, Unconjugated (bs-2734R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Bcl-6 Polyclonal Antibody, Unconjugated (bs-2734R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Bcl-6 Polyclonal Antibody, Unconjugated (bs-2734R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.