bs-2735R [Primary Antibody]
GST Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: GSTp1

Specificity: GSTp2 in mouse

Immunogen Range: 121-210/210

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 14870

Swiss Prot: P19157

Source: KLH conjugated synthetic peptide derived from Mouse GstP1

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage: Store at -20°C for 12 months..

Background: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Can metabolize 1-chloro-2,4-dinitrobenzene. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration (By similarity).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-1000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 23

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhang, Fu, et al. "Up-regulation of protein tyrosine nitration in methamphetamine-induced neurotoxicity through DDAH/ADMA/NOS pathway." Neurochemistry International (2013).Read more>>
  • Li, Hongyan, et al. "Differential proteome and gene expression reveal response to carbon ion irradiation in pubertal mice testes." Toxicology Letters (2014).Read more>>
  • Zhang, Chunlei, et al. "Insights into the Distinguishing Stress-induced Cytotoxicity of Chiral Gold Nanoclusters and the Relationship with GSTP1." TheranosticsRead more>>
  • Lin et al. Penehyclidine Hydrochloride Preconditioning Provides Cardioprotection in a Rat Model of Myocardial Ischemia/Reperfusion Injury. (2015) PLoS.One. 10:e0138051Read more>>
  • Jiang et al. Scavenger receptor-C acts as a receptor for Bacillus thuringiensis vegetative insecticidal protein Vip3Aa and mediates the internalization of Vip3Aa via endocytosis. (2018) PLoS.Pathog. 14:e1007347Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human kidney labeled with Anti-GST Polyclonal Antibody, Unconjugated (bs-2735R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Mouse splenocytes probed with Rabbit Anti-GST Polyclonal Antibody, Unconjugated (bs-2735R) (green) at 1:100 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only(light blue), and isotype control(orange).


Paraformaldehyde-fixed, paraffin embedded (Rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal rabbit serum) at 37°C for 30min; Incubation: Anti-GST Antibody, conjugated (bs-2735R-AF594) at 1:200 overnight at 4°C; DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded (Mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal rabbit serum) at 37°C for 30min; Incubation: Anti-GST Antibody, conjugated (bs-2735R-AF594) at 1:200 overnight at 4°C; DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded (Mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal rabbit serum) at 37°C for 30min; Incubation: Anti-GST Antibody, conjugated (bs-2735R-AF594) at 1:200 overnight at 4°C; DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded (Mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal rabbit serum) at 37°C for 30min; Incubation: Anti-GST Antibody, conjugated (bs-2735R-AF594) at 1:200 overnight at 4°C; DAPI (blue, C02-04002) was used to stain the cell nuclei.