bs-2736R-FITC [Conjugated Primary Antibody]
IRS-1(Ser307) Polyclonal Antibody, FITC Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: IRS-1 Ser307

Specificity: This phosphorylation site is homologous to Ser302 in Mouse and Rat.

Modification Site: Ser307

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3667

Swiss Prot: P35568

Source: KLH conjugated synthetic phosphopeptide derived from human IRS1 around the phosphorylation site of Ser307 [TE(p-S)IT]

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity).

Conjugation: FITC

Excitation/ Emission: 494nm/518nm

Size: 100ul

Concentration: 1ug/ul

Applications: FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 132


Cross Reactive Species: Human
Mouse
Rat
Goat

Predicted Cross Reactive Species: Dog
Cow
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Luo, Cheng, et al. "Kaempferol alleviates insulin resistance via hepatic IKK/NF-κB signal in type 2 diabetic rats." International Immunopharmacology 28.1 (2015): 744-750.Read more>>
  • Gao, Sujie, et al. "Propofol inhibits growth of neurons through regulating insulin receptor and insulin-like growth factor-1 receptor." Int J Clin Exp Pathol 9.7 (2016): 6785-6794.Read more>>
  • Zhang et al. Astragalus Polysaccharide Improves Insulin Sensitivity via AMPK Activation in 3T3-L1 Adipocytes. (2018) Molecules. 23 Read more>>
  • Zhang T et al. Dietary Sea Buckthorn Pomace Induces Beige Adipocyte Formation in Inguinal White Adipose Tissue in Lambs. Animals (Basel). 2019 Apr 24;9(4). pii: E193.Read more>>
  • Yang Hui. et al. Activation of Hippocampal IR/IRS-1 Signaling Contributes to the Treatment with Zuogui Jiangtang Jieyu Decoction on the Diabetes-Related Depression. Evid-Based Compl Alt. 2021;2021:6688723Read more>>
  • Wang, Hongyan. et al. Protection of pancreatic -cell by phosphocreatine through mitochondrial improvement via the regulation of dual AKT/IRS-1/GSK-3 and STAT3/Cyp-D signaling pathways. 2021 Aug 28Read more>>
VALIDATION IMAGES